Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary.
FEMS Microbiol Lett. 2009 Nov;300(1):58-67. doi: 10.1111/j.1574-6968.2009.01765.x. Epub 2009 Aug 21.
Green mold of Pleurotus ostreatus, caused by Trichoderma species, has recently resulted in crop losses worldwide. Therefore, there is an emerging need for rapid means of diagnosing the causal agents. A PCR assay was developed for rapid detection of Trichoderma pleurotum and Trichoderma pleuroticola, the two pathogens causing green mold of P. ostreatus. Three oligonucleotide primers were designed for identifying these species in a multiplex PCR assay based on DNA sequences within the fourth and fifth introns in the translation elongation factor 1alpha gene. The primers detected the presence of T. pleurotum and/or T. pleuroticola directly in the growing substrates of oyster mushrooms, without the need for isolating the pathogens. The assay was used to assess the presence of the two species in natural environments in which P. ostreatus can be found in Hungary, and demonstrated that T. pleuroticola was present in the growing substrates and on the surface of the basidiomes of wild oyster mushrooms. Other Trichoderma species detected in these substrates and habitats were Trichoderma harzianum, Trichoderma longibrachiatum and Trichoderma atroviride. Trichoderma pleurotum was not found in any of the samples from the forested areas tested in this study.
绿霉病由木霉属真菌引起，最近已导致全球范围内的作物损失。因此，迫切需要快速诊断致病因子的方法。本研究开发了一种用于快速检测导致平菇绿霉病的木霉属真菌（绿僵菌和绿粘帚霉）的 PCR 检测方法。基于翻译延伸因子 1α 基因的第四和第五内含子中的 DNA 序列，设计了三个寡核苷酸引物，用于在多重 PCR 检测中鉴定这些物种。这些引物可直接在双孢蘑菇生长基质中检测到绿僵菌和/或绿粘帚霉的存在，而无需分离病原菌。该检测方法用于评估在匈牙利可发现平菇的自然环境中这两个物种的存在情况，结果表明绿粘帚霉存在于生长基质中和野生双孢蘑菇菌盖上。在这些基质和栖息地中检测到的其他木霉属真菌包括哈茨木霉、长枝木霉和深绿木霉。在本研究中检测的森林地区的任何样本中均未发现绿僵菌。
