Sannino A, Madaghiele M, Conversano F, Mele G, Maffezzoli A, Netti P A, Ambrosio L, Nicolais L
Department of Innovation Engineering, University of Lecce, via Monteroni, 73100 Lecce, Italy.
Biomacromolecules. 2004 Jan-Feb;5(1):92-6. doi: 10.1021/bm0341881.
The aim of this work is to obtain a chemically cross-linked hydrogel from hyaluronic acid and cellulose derivatives that exhibits sensitivity to variation of the composition of the external absorbing medium and an equilibrium sorption capacity higher than a common hyaluronic acid-based hydrogel, in view of its potential use in prevention of postsurgical soft tissue adhesion. This has been achieved by chemical stabilization of hyaluronic acid (HA) and cellulose derivatives, hydroxyethylcellulose (HEC) and carboxymethylcellulose (CMCNa) through the difunctional cross-linker divinyl sulfone. Significant increase in sorption capacity, both in water and in water solutions at different ionic strength, has been observed for these samples in comparison with hydrogels obtained through chemical stabilization of hyaluronic acid. Moreover, different dehydration procedures adopted for the xerogel synthesis have been used, which resulted in a modulation of the equilibrium sorption capacity. Hyaluronic acid stability has been confirmed by means of NMR analysis.
这项工作的目的是从透明质酸和纤维素衍生物中获得一种化学交联水凝胶，鉴于其在预防术后软组织粘连方面的潜在用途，该水凝胶对外吸收介质组成的变化表现出敏感性，并且平衡吸附能力高于普通的基于透明质酸的水凝胶。这是通过双官能交联剂二乙烯基砜对透明质酸（HA）、纤维素衍生物羟乙基纤维素（HEC）和羧甲基纤维素（CMCNa）进行化学稳定来实现的。与通过透明质酸化学稳定获得的水凝胶相比，这些样品在水和不同离子强度的水溶液中的吸附能力都有显著提高。此外，采用了不同的干凝胶合成脱水程序，这导致了平衡吸附能力的调节。通过核磁共振分析证实了透明质酸的稳定性。
