开发一种用于快速检测马抗马巴贝斯虫抗体的重组EMA-2免疫层析检测方法。
Development of an immunochromatographic test with recombinant EMA-2 for the rapid detection of antibodies against Babesia equi in horses.
作者信息
Huang Xiaohong, Xuan Xuenan, Xu Longshan, Zhang Shoufa, Yokoyama Naoaki, Suzuki Naoyoshi, Igarashi Ikuo
机构信息
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.
出版信息
J Clin Microbiol. 2004 Jan;42(1):359-61. doi: 10.1128/JCM.42.1.359-361.2004.
Abstract
An immunochromatographic test (BeICT) for the rapid detection of antibodies against Babesia equi was developed. It clearly differentiated B. equi-infected horses from B. caballi-infected and uninfected horses. The agreement with enzyme-linked immunosorbent assay results was 96.7% in the detection of field sera. The results suggest that BeICT is rapid, simple, reliable, and suitable for use to detect B. equi infection in the field.
摘要
开发了一种用于快速检测抗马巴贝斯虫抗体的免疫层析试验（BeICT）。它能清晰地区分感染马巴贝斯虫的马与感染驽巴贝斯虫的马以及未感染的马。在检测现场血清时，其与酶联免疫吸附测定结果的一致性为96.7%。结果表明，BeICT快速、简便、可靠，适用于现场检测马巴贝斯虫感染。
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