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Strengths and limitations of commercial tests for hepatitis C virus RNA quantification.丙型肝炎病毒RNA定量商业检测的优势与局限性
J Clin Microbiol. 2004 Jan;42(1):421-5. doi: 10.1128/JCM.42.1.421-425.2004.
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本文引用的文献

1
Comparison of three commercially available assays for HCV RNA using the international unit standard: implications for management of patients with chronic hepatitis C virus infection in clinical practice.使用国际单位标准对三种市售丙型肝炎病毒RNA检测方法进行比较：对慢性丙型肝炎病毒感染患者临床治疗的意义。
Am J Gastroenterol. 2003 May;98(5):1159-66. doi: 10.1111/j.1572-0241.2003.07449.x.
2
Peginterferon alfa-2a plus ribavirin for chronic hepatitis C virus infection.聚乙二醇干扰素α-2a联合利巴韦林治疗慢性丙型肝炎病毒感染
N Engl J Med. 2002 Sep 26;347(13):975-82. doi: 10.1056/NEJMoa020047.
3
Comparison of methodologies for quantification of hepatitis C virus (HCV) RNA in patients coinfected with HCV and human immunodeficiency virus.丙型肝炎病毒（HCV）与人类免疫缺陷病毒合并感染患者中HCV RNA定量方法的比较
Clin Infect Dis. 2002 Aug 15;35(4):482-7. doi: 10.1086/341976. Epub 2002 Jul 22.
4
Evaluation of the VERSANT HCV RNA 3.0 assay for quantification of hepatitis C virus RNA in serum.VERSANT丙型肝炎病毒RNA 3.0检测法用于血清中丙型肝炎病毒RNA定量的评估。
J Clin Microbiol. 2002 Jun;40(6):2031-6. doi: 10.1128/JCM.40.6.2031-2036.2002.
5
Performance of the New Bayer VERSANT HCV RNA 3.0 assay for quantitation of hepatitis C virus RNA in plasma and serum: conversion to international units and comparison with the Roche COBAS Amplicor HCV Monitor, Version 2.0, assay.新型拜耳VERSANT HCV RNA 3.0检测法用于定量检测血浆和血清中丙型肝炎病毒RNA的性能：转换为国际单位并与罗氏COBAS Amplicor HCV Monitor 2.0版检测法进行比较。
J Clin Microbiol. 2002 Mar;40(3):788-93. doi: 10.1128/JCM.40.3.788-793.2002.
6
Performance characteristics of the COBAS Amplicor Hepatitis C Virus (HCV) Monitor, Version 2.0, International Unit assay and the National Genetics Institute HCV Superquant assay.COBAS Amplicor丙型肝炎病毒（HCV）监测仪2.0版国际单位检测法与国家基因研究所HCV超定量检测法的性能特征。
J Clin Microbiol. 2002 Mar;40(3):768-73. doi: 10.1128/JCM.40.3.768-773.2002.
7
Comparative evaluation of the VERSANT HCV RNA 3.0, QUANTIPLEX HCV RNA 2.0, and COBAS AMPLICOR HCV MONITOR version 2.0 Assays for quantification of hepatitis C virus RNA in serum.VERSANT HCV RNA 3.0、QUANTIPLEX HCV RNA 2.0和COBAS AMPLICOR HCV MONITOR 2.0检测法用于血清中丙型肝炎病毒RNA定量的比较评估。
J Clin Microbiol. 2002 Feb;40(2):495-500. doi: 10.1128/JCM.40.2.495-500.2002.
8
Peginterferon alfa-2b plus ribavirin compared with interferon alfa-2b plus ribavirin for initial treatment of chronic hepatitis C: a randomised trial.聚乙二醇干扰素α-2b联合利巴韦林与干扰素α-2b联合利巴韦林用于初治慢性丙型肝炎的比较：一项随机试验
Lancet. 2001 Sep 22;358(9286):958-65. doi: 10.1016/s0140-6736(01)06102-5.
9
Improved version 2.0 qualitative and quantitative AMPLICOR reverse transcription-PCR tests for hepatitis C virus RNA: calibration to international units, enhanced genotype reactivity, and performance characteristics.丙型肝炎病毒RNA的改进版2.0定性和定量AMPLICOR逆转录-聚合酶链反应检测：国际单位校准、增强的基因型反应性及性能特征
J Clin Microbiol. 2000 Nov;38(11):4171-9. doi: 10.1128/JCM.38.11.4171-4179.2000.
10
Performance characteristics of the QUANTIPLEX HIV-1 RNA 3.0 assay for detection and quantitation of human immunodeficiency virus type 1 RNA in plasma.用于检测和定量血浆中人类免疫缺陷病毒1型RNA的QUANTIPLEX HIV-1 RNA 3.0检测法的性能特征
J Clin Microbiol. 2000 Aug;38(8):2837-45. doi: 10.1128/JCM.38.8.2837-2845.2000.

丙型肝炎病毒RNA定量商业检测的优势与局限性

Strengths and limitations of commercial tests for hepatitis C virus RNA quantification.

作者信息

Morishima Chihiro, Chung Minjun, Ng Ka Wing, Brambilla Donald J, Gretch David R

机构信息

Department of Pediatrics, University of Washington School of Medicine, Seattle, Washington, USA.

出版信息

J Clin Microbiol. 2004 Jan;42(1):421-5. doi: 10.1128/JCM.42.1.421-425.2004.

DOI:10.1128/JCM.42.1.421-425.2004
PMID:14715796
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC321650/
Abstract

The sensitivity of the Roche COBAS Monitor v.2.0 test was slightly better than that of the Bayer bDNA 3.0 test, but the Monitor test underquantified specimens by 2.5- to 10.6-fold even at relatively low hepatitis C virus RNA concentrations. Dilution prior to assay minimally decreased the reproducibility of the Monitor assay, leading to the recommendation for all specimens to be diluted 1:100 prior to testing by this assay.

摘要

罗氏COBAS Monitor v.2.0检测的灵敏度略高于拜耳bDNA 3.0检测，但即使在丙型肝炎病毒RNA浓度相对较低时，Monitor检测也会使样本定量偏低2.5至10.6倍。检测前稀释对Monitor检测的可重复性影响极小，因此建议在使用该检测方法进行检测前，将所有样本按1:100进行稀释。