丙型肝炎病毒RNA定量商业检测的优势与局限性
Strengths and limitations of commercial tests for hepatitis C virus RNA quantification.
作者信息
Morishima Chihiro, Chung Minjun, Ng Ka Wing, Brambilla Donald J, Gretch David R
机构信息
Department of Pediatrics, University of Washington School of Medicine, Seattle, Washington, USA.
出版信息
J Clin Microbiol. 2004 Jan;42(1):421-5. doi: 10.1128/JCM.42.1.421-425.2004.
Abstract
The sensitivity of the Roche COBAS Monitor v.2.0 test was slightly better than that of the Bayer bDNA 3.0 test, but the Monitor test underquantified specimens by 2.5- to 10.6-fold even at relatively low hepatitis C virus RNA concentrations. Dilution prior to assay minimally decreased the reproducibility of the Monitor assay, leading to the recommendation for all specimens to be diluted 1:100 prior to testing by this assay.
摘要
罗氏COBAS Monitor v.2.0检测的灵敏度略高于拜耳bDNA 3.0检测，但即使在丙型肝炎病毒RNA浓度相对较低时，Monitor检测也会使样本定量偏低2.5至10.6倍。检测前稀释对Monitor检测的可重复性影响极小，因此建议在使用该检测方法进行检测前，将所有样本按1:100进行稀释。
相似文献
1
Strengths and limitations of commercial tests for hepatitis C virus RNA quantification.丙型肝炎病毒RNA定量商业检测的优势与局限性
J Clin Microbiol. 2004 Jan;42(1):421-5. doi: 10.1128/JCM.42.1.421-425.2004.
2
Comparative evaluation of hepatitis C virus RNA quantitation by branched DNA, NASBA, and monitor assays.通过分支DNA、核酸序列扩增法和监测检测法对丙型肝炎病毒RNA定量进行比较评估。
Hepatology. 1999 Feb;29(2):528-35. doi: 10.1002/hep.510290237.
3
Clinical evaluation of the automated COBAS AMPLICOR HCV MONITOR test version 2.0 for quantifying serum hepatitis C virus RNA and comparison to the quantiplex HCV version 2.0 test.用于定量检测血清丙型肝炎病毒RNA的COBAS AMPLICOR HCV MONITOR 2.0版自动检测方法的临床评估以及与Quantiplex HCV 2.0版检测方法的比较
J Clin Microbiol. 2000 Aug;38(8):2933-9. doi: 10.1128/JCM.38.8.2933-2939.2000.
4
Evaluation of the VERSANT HCV RNA 3.0 assay for quantification of hepatitis C virus RNA in serum.VERSANT丙型肝炎病毒RNA 3.0检测法用于血清中丙型肝炎病毒RNA定量的评估。
J Clin Microbiol. 2002 Jun;40(6):2031-6. doi: 10.1128/JCM.40.6.2031-2036.2002.
5
Multicenter comparison of Roche COBAS AMPLICOR MONITOR version 1.5, Organon Teknika NucliSens QT with Extractor, and Bayer Quantiplex version 3.0 for quantification of human immunodeficiency virus type 1 RNA in plasma.罗氏COBAS AMPLICOR MONITOR 1.5版、配有提取仪的奥加农Technika NucliSens QT以及拜耳Quantiplex 3.0版用于血浆中人类免疫缺陷病毒1型RNA定量的多中心比较
J Clin Microbiol. 2000 Nov;38(11):4034-41. doi: 10.1128/JCM.38.11.4034-4041.2000.
6
Performance of the New Bayer VERSANT HCV RNA 3.0 assay for quantitation of hepatitis C virus RNA in plasma and serum: conversion to international units and comparison with the Roche COBAS Amplicor HCV Monitor, Version 2.0, assay.新型拜耳VERSANT HCV RNA 3.0检测法用于定量检测血浆和血清中丙型肝炎病毒RNA的性能：转换为国际单位并与罗氏COBAS Amplicor HCV Monitor 2.0版检测法进行比较。
J Clin Microbiol. 2002 Mar;40(3):788-93. doi: 10.1128/JCM.40.3.788-793.2002.
7
Performance characteristics of the COBAS AMPLICOR hepatitis C virus MONITOR Test, version 2.0.COBAS AMPLICOR丙型肝炎病毒监测检测2.0版的性能特征。
Am J Clin Pathol. 2000 Aug;114(2):180-7. doi: 10.1309/KAJF-DL9G-0PNB-0FYH.
8
9
Comparison of qualitative (COBAS AMPLICOR HCV 2.0 versus VERSANT HCV RNA) and quantitative (COBAS AMPLICOR HCV monitor 2.0 versus VERSANT HCV RNA 3.0) assays for hepatitis C virus (HCV) RNA detection and quantification: impact on diagnosis and treatment of HCV infections.丙型肝炎病毒（HCV）RNA检测和定量的定性（COBAS AMPLICOR HCV 2.0与VERSANT HCV RNA）及定量（COBAS AMPLICOR HCV monitor 2.0与VERSANT HCV RNA 3.0）检测方法的比较：对HCV感染诊断和治疗的影响
J Clin Microbiol. 2005 Jun;43(6):2590-7. doi: 10.1128/JCM.43.6.2590-2597.2005.
10
Comparison of performance characteristics of three real-time reverse transcription-PCR test systems for detection and quantification of hepatitis C virus.三种用于丙型肝炎病毒检测和定量的实时逆转录-聚合酶链反应检测系统的性能特征比较
J Clin Microbiol. 2007 Aug;45(8):2529-36. doi: 10.1128/JCM.00058-07. Epub 2007 Jun 13.
引用本文的文献
1
Biological and nanotechnological applications using interactions between ionic liquids and nucleic acids.利用离子液体与核酸之间的相互作用的生物和纳米技术应用。
Biophys Rev. 2018 Jun;10(3):931-940. doi: 10.1007/s12551-018-0422-7. Epub 2018 Apr 23.
2
Vitamin D Status and Virologic Response to HCV Therapy in the HALT-C and VIRAHEP-C Trials.在HALT-C和VIRAHEP-C试验中维生素D状态与丙型肝炎病毒治疗的病毒学反应
PLoS One. 2016 Nov 10;11(11):e0166036. doi: 10.1371/journal.pone.0166036. eCollection 2016.
3
Structure, stability and behaviour of nucleic acids in ionic liquids.离子液体中核酸的结构、稳定性及行为
Nucleic Acids Res. 2014 Aug;42(14):8831-44. doi: 10.1093/nar/gku499. Epub 2014 Jul 10.
4
Processes to manage analyses and publications in a phase III multicenter randomized clinical trial.在一项III期多中心随机临床试验中管理分析和发表的流程。
Trials. 2014 May 7;15:159. doi: 10.1186/1745-6215-15-159.
5
Reduction in Hepatic Inflammation Is Associated With Less Fibrosis Progression and Fewer Clinical Outcomes in Advanced Hepatitis C.肝内炎症减轻与晚期丙型肝炎患者的纤维化进展减缓及临床结局改善相关。
Am J Gastroenterol. 2012 Sep;107(9):1388-98. doi: 10.1038/ajg.2012.137. Epub 2012 Jun 12.
6
Coffee consumption is associated with response to peginterferon and ribavirin therapy in patients with chronic hepatitis C.咖啡饮用与慢性丙型肝炎患者对聚乙二醇干扰素和利巴韦林治疗的反应相关。
Gastroenterology. 2011 Jun;140(7):1961-9. doi: 10.1053/j.gastro.2011.02.061. Epub 2011 Mar 2.
7
Performance of the Abbott real-time PCR assay using m2000sp and m2000rt for hepatitis C virus RNA quantification.使用m2000sp和m2000rt的雅培实时荧光定量PCR检测法对丙型肝炎病毒RNA进行定量分析的性能。
J Clin Microbiol. 2009 Jun;47(6):1726-32. doi: 10.1128/JCM.01300-08. Epub 2009 Apr 15.
8
A novel diagnostic target in the hepatitis C virus genome.丙型肝炎病毒基因组中的一个新的诊断靶点。
PLoS Med. 2009 Feb 10;6(2):e31. doi: 10.1371/journal.pmed.1000031.
9
Interpretation of positive transcription-mediated amplification test results from polymerase chain reaction-negative samples obtained after treatment of chronic hepatitis C.慢性丙型肝炎治疗后聚合酶链反应阴性样本的转录介导扩增试验阳性结果解读
Hepatology. 2008 Nov;48(5):1412-9. doi: 10.1002/hep.22487.
10
Comparison of performance characteristics of three real-time reverse transcription-PCR test systems for detection and quantification of hepatitis C virus.三种用于丙型肝炎病毒检测和定量的实时逆转录-聚合酶链反应检测系统的性能特征比较
J Clin Microbiol. 2007 Aug;45(8):2529-36. doi: 10.1128/JCM.00058-07. Epub 2007 Jun 13.
本文引用的文献
1
Comparison of three commercially available assays for HCV RNA using the international unit standard: implications for management of patients with chronic hepatitis C virus infection in clinical practice.使用国际单位标准对三种市售丙型肝炎病毒RNA检测方法进行比较：对慢性丙型肝炎病毒感染患者临床治疗的意义。
Am J Gastroenterol. 2003 May;98(5):1159-66. doi: 10.1111/j.1572-0241.2003.07449.x.
2
Peginterferon alfa-2a plus ribavirin for chronic hepatitis C virus infection.聚乙二醇干扰素α-2a联合利巴韦林治疗慢性丙型肝炎病毒感染
N Engl J Med. 2002 Sep 26;347(13):975-82. doi: 10.1056/NEJMoa020047.
3
Comparison of methodologies for quantification of hepatitis C virus (HCV) RNA in patients coinfected with HCV and human immunodeficiency virus.丙型肝炎病毒（HCV）与人类免疫缺陷病毒合并感染患者中HCV RNA定量方法的比较
Clin Infect Dis. 2002 Aug 15;35(4):482-7. doi: 10.1086/341976. Epub 2002 Jul 22.
4
Evaluation of the VERSANT HCV RNA 3.0 assay for quantification of hepatitis C virus RNA in serum.VERSANT丙型肝炎病毒RNA 3.0检测法用于血清中丙型肝炎病毒RNA定量的评估。
J Clin Microbiol. 2002 Jun;40(6):2031-6. doi: 10.1128/JCM.40.6.2031-2036.2002.
5
Performance of the New Bayer VERSANT HCV RNA 3.0 assay for quantitation of hepatitis C virus RNA in plasma and serum: conversion to international units and comparison with the Roche COBAS Amplicor HCV Monitor, Version 2.0, assay.新型拜耳VERSANT HCV RNA 3.0检测法用于定量检测血浆和血清中丙型肝炎病毒RNA的性能：转换为国际单位并与罗氏COBAS Amplicor HCV Monitor 2.0版检测法进行比较。
J Clin Microbiol. 2002 Mar;40(3):788-93. doi: 10.1128/JCM.40.3.788-793.2002.
6
Performance characteristics of the COBAS Amplicor Hepatitis C Virus (HCV) Monitor, Version 2.0, International Unit assay and the National Genetics Institute HCV Superquant assay.COBAS Amplicor丙型肝炎病毒（HCV）监测仪2.0版国际单位检测法与国家基因研究所HCV超定量检测法的性能特征。
J Clin Microbiol. 2002 Mar;40(3):768-73. doi: 10.1128/JCM.40.3.768-773.2002.
7
Comparative evaluation of the VERSANT HCV RNA 3.0, QUANTIPLEX HCV RNA 2.0, and COBAS AMPLICOR HCV MONITOR version 2.0 Assays for quantification of hepatitis C virus RNA in serum.VERSANT HCV RNA 3.0、QUANTIPLEX HCV RNA 2.0和COBAS AMPLICOR HCV MONITOR 2.0检测法用于血清中丙型肝炎病毒RNA定量的比较评估。
J Clin Microbiol. 2002 Feb;40(2):495-500. doi: 10.1128/JCM.40.2.495-500.2002.
8
Peginterferon alfa-2b plus ribavirin compared with interferon alfa-2b plus ribavirin for initial treatment of chronic hepatitis C: a randomised trial.聚乙二醇干扰素α-2b联合利巴韦林与干扰素α-2b联合利巴韦林用于初治慢性丙型肝炎的比较：一项随机试验
Lancet. 2001 Sep 22;358(9286):958-65. doi: 10.1016/s0140-6736(01)06102-5.
9
Improved version 2.0 qualitative and quantitative AMPLICOR reverse transcription-PCR tests for hepatitis C virus RNA: calibration to international units, enhanced genotype reactivity, and performance characteristics.丙型肝炎病毒RNA的改进版2.0定性和定量AMPLICOR逆转录-聚合酶链反应检测：国际单位校准、增强的基因型反应性及性能特征
J Clin Microbiol. 2000 Nov;38(11):4171-9. doi: 10.1128/JCM.38.11.4171-4179.2000.
10
Performance characteristics of the QUANTIPLEX HIV-1 RNA 3.0 assay for detection and quantitation of human immunodeficiency virus type 1 RNA in plasma.用于检测和定量血浆中人类免疫缺陷病毒1型RNA的QUANTIPLEX HIV-1 RNA 3.0检测法的性能特征
J Clin Microbiol. 2000 Aug;38(8):2837-45. doi: 10.1128/JCM.38.8.2837-2845.2000.
Zotero 插件