Siwek M, Buitenhuis A J, Cornelissen S J B, Nieuwland M G B, Bovenhuis H, Crooijmans R P M A, Groenen M A M, de Vries-Reilingh G, Parmentier H K, van der Poel J J
Animal Breeding & Genetics Group, Wageningen Institute of Animal Sciences, Wageningen University, PO Box 338, 6700 AH Wageningen, The Netherlands.
Poult Sci. 2003 Dec;82(12):1845-52. doi: 10.1093/ps/82.12.1845.
Quantitative trait loci involved in the primary antibody response to keyhole lympet hemocyanin (KLH) and Mycobacterium butyricum were detected in two independent populations of laying hens. The first population was an F2 cross (H/L) of lines divergently selected for either high or low primary antibody responses to SRBC, and the second population was an F2 cross between 2 commercial layer lines displaying differences in feather pecking behavior (FP). Both populations were typed with microsatellite markers widely distributed over the genome with similar intervals between markers. Titers of antibodies binding KLH and M. butyricum were measured for all individuals by ELISA. Two genetic models were applied to detect QTL involved in the humoral immune response: a half-sib model and a line-cross model, both using the regression interval method. In the half-sib analysis, 2 QTL (on GGA14 and GGA27) were detected for the antibody response to KLH for the H/L population, and 2 QTL (on GGA14 and GGA18) were detected for the FP population. Only 1 QTL was detected for M. butyricum on GGA14 in the FP population using the half-sib analysis model. Two QTL were detected for the FP population on GGA2 and GGA3 using the line-cross analysis model. A QTL for the primary antibody response to KLH detected on GGA14 was validated in both populations under the half-sib analysis model. The present data suggest differences in the genetic regulation of antibody responses to two different T-cell dependent antigens.
在两个独立的蛋鸡群体中检测到了参与对钥孔戚血蓝蛋白（KLH）和丁酸分枝杆菌的初次抗体反应的数量性状位点。第一个群体是对SRBC的初次抗体反应被分别选择为高或低的品系的F2杂交（H/L）群体，第二个群体是在啄羽行为（FP）上表现出差异的两个商业蛋鸡品系之间的F2杂交群体。两个群体都用在基因组上广泛分布且标记之间间隔相似的微卫星标记进行分型。通过ELISA测量所有个体结合KLH和丁酸分枝杆菌的抗体滴度。应用两种遗传模型来检测参与体液免疫反应的QTL：半同胞模型和品系杂交模型，两者都使用回归区间法。在半同胞分析中，对于H/L群体，检测到2个与对KLH的抗体反应相关的QTL（位于GGA14和GGA27上），对于FP群体，检测到2个QTL（位于GGA14和GGA18上）。使用半同胞分析模型，在FP群体中仅在GGA14上检测到1个与对丁酸分枝杆菌的反应相关的QTL。使用品系杂交分析模型，在GGA2和GGA3上为FP群体检测到2个QTL。在半同胞分析模型下，在两个群体中都验证了在GGA14上检测到的与对KLH的初次抗体反应相关的QTL。目前的数据表明对两种不同的T细胞依赖性抗原的抗体反应的遗传调控存在差异。
