Siwek M, Slawinska A, Rydzanicz M, Wesoly J, Fraszczak M, Suchocki T, Skiba J, Skiba K, Szyda J
Animal Biotechnology Department, University of Technology and Life Sciences, Mazowiecka 28, 84-085, Bydgoszcz, Poland.
Anim Genet. 2015 Jun;46(3):247-54. doi: 10.1111/age.12280. Epub 2015 Mar 5.
There are two categories of immune responses - innate and adaptive immunity - both having polygenic backgrounds and a significant environmental component. In our study, adaptive immunity was represented by the specific antibody response toward keyhole limpet hemocyanin (KLH); innate immunity was represented by natural antibodies toward lipopolysaccharide (LPS) and lipoteichoic acid (LTA). Defining genetic bases of immune responses leads from defining quantitative trait loci (QTL) toward a single mutation responsible for variation in the phenotypic trait. The goal of the reported study was to define candidate genes and mutations for the immune traits of interest in chicken by performing an association study of SNPs located in candidate genes defined in QTL regions. Candidate genes and SNPs in QTL regions were selected in silico. SNP association was based on a custom SNP panel, GoldenGate genotyping assay (Illumina) and two statistical models: random mixed model and CAR score. The most significant SNP for immune response toward KLH was located in the JMJD6 gene located on GGA18. Four SNPs in candidate genes FOXJ1 (GGA18), EPHB1 (GGA9), PTGER4 (GGAZ) and PRKCB (GGA14) showed association with natural antibodies for LPS. A single SNP in ITGB4 (GGA18) was associated with natural antibodies for LTA. All associated SNPs mentioned above showed additive effects.
免疫反应有两类——先天免疫和适应性免疫，两者都具有多基因背景和重要的环境因素。在我们的研究中，适应性免疫由针对钥孔血蓝蛋白（KLH）的特异性抗体反应来体现；先天免疫由针对脂多糖（LPS）和脂磷壁酸（LTA）的天然抗体来体现。确定免疫反应的遗传基础是从确定数量性状基因座（QTL）开始，进而找到导致表型性状变异的单个突变。本报告研究的目标是通过对位于QTL区域所定义的候选基因中的单核苷酸多态性（SNP）进行关联研究，来确定鸡感兴趣的免疫性状的候选基因和突变。在计算机上选择QTL区域中的候选基因和SNP。SNP关联基于定制的SNP芯片、金标准基因分型检测（Illumina）以及两种统计模型：随机混合模型和CAR评分。对KLH免疫反应最显著的SNP位于GGA18上的JMJD6基因中。候选基因FOXJ1（GGA18）、EPHB1（GGA9）、PTGER4（GGAZ）和PRKCB（GGA14）中的四个SNP显示与针对LPS的天然抗体有关联。ITGB4（GGA18）中的一个SNP与针对LTA的天然抗体有关联。上述所有相关SNP均显示出加性效应。
