Deaton Christopher M, Marlin David J, Smith Nicola C, Harris Pat A, Roberts Colin A, Schroter Robert C, Kelly Frank J
Centre for Equine Studies, Animal Health Trust, Lanwades Park, Kentford, Suffolk, CB8 7UU, UK.
Am J Vet Res. 2004 Jan;65(1):80-7. doi: 10.2460/ajvr.2004.65.80.
To determine the pulmonary epithelial lining fluid (ELF) concentrations and degree of oxidation of ascorbic acid in horses affected by recurrent airway obstruction (RAO) in the presence and absence of neutrophilic airway inflammation.
6 RAO-affected horses and 8 healthy control horses.
Nonenzymatic antioxidant concentrations were determined in RBC, plasma, and ELF samples of control horses and RAO-affected horses in the presence and absence of airway inflammation.
ELF ascorbic acid concentration was decreased in RAO-affected horses with airway inflammation (median, 0.06 mmol/L; 25th and 75th percentiles, 0.0 and 0.4 mmol/L), compared with RAO-affected horses without airway inflammation (1.0 mmol/L; 0.7 and 1.5 mmol/L) and control horses (2.2 mmol/L; 1.4 and 2.2 mmol/L). Epithelial lining fluid ascorbic acid remained significantly lower in RAO-affected horses without airway inflammation than in control horses. Moreover, the ELF ascorbic acid redox ratio (ie, ratio of the concentrations of dehydroascorbate to total ascorbic acid) was higher in RAO-affected horses with airway inflammation (median, 0.85; 25th and 75th percentiles, 0.25 and 1.00), compared with RAO-affected horses without airway inflammation (0.04; 0.02 and 0.22). The number of neutrophils in bronchoalveolar lavage fluid was inversely related to the ELF ascorbic acid concentration (r = -0.81) and positively correlated with the ascorbic acid redox ratio (r = 0.65).
Neutrophilic inflammation in horses affected by RAO is associated with a reduction in the ELF ascorbic acid pool. Nutritional supplementation with ascorbic acid derivatives in horses affected by RAO is an area for further investigation.
确定在有和没有嗜中性气道炎症的情况下，患复发性气道阻塞（RAO）马匹的肺上皮衬液（ELF）中抗坏血酸的浓度及其氧化程度。
6匹患RAO的马和8匹健康对照马。
在有和没有气道炎症的情况下，测定对照马和患RAO马的红细胞、血浆和ELF样本中的非酶抗氧化剂浓度。
与没有气道炎症的患RAO马（1.0 mmol/L；第25和第75百分位数，0.7和1.5 mmol/L）以及对照马（2.2 mmol/L；第25和第75百分位数，1.4和2.2 mmol/L）相比，有气道炎症的患RAO马的ELF抗坏血酸浓度降低（中位数，0.06 mmol/L；第25和第75百分位数，0.0和0.4 mmol/L）。没有气道炎症的患RAO马的上皮衬液抗坏血酸浓度仍显著低于对照马。此外，与没有气道炎症的患RAO马（0.04；第25和第75百分位数，0.02和0.22）相比，有气道炎症的患RAO马的ELF抗坏血酸氧化还原比（即脱氢抗坏血酸与总抗坏血酸浓度之比）更高（中位数，0.85；第25和第75百分位数，0.25和1.00）。支气管肺泡灌洗液中的嗜中性粒细胞数量与ELF抗坏血酸浓度呈负相关（r = -0.81），与抗坏血酸氧化还原比呈正相关（r = 0.65）。
患RAO马匹的嗜中性炎症与ELF抗坏血酸池的减少有关。对患RAO马匹补充抗坏血酸衍生物营养是一个有待进一步研究的领域。
