Su Vincent, Hsu Ban-Dar
Department of Life Science, National Tsing Hua University, Hsin-Chu 30055, Taiwan.
Biotechnol Lett. 2003 Nov;25(22):1933-9. doi: 10.1023/b:bile.0000003989.19657.53.
Anthocyanins are responsible for reds through blues in flowers. Blue and violet flowers generally contain derivatives of delphinidin, whereas red and pink flowers contain derivatives of cyanidin or pelargonidin. Differences in hydroxylation patterns of these three major classes of anthocyanidins are controlled by the cytochrome P450 enzymes. Flavonoid-3',5'-hydroxylase, a member of the cytochrome P450 family, is the key enzyme in the synthesis of 3',5'-hydroxylated anthocyanins, generally required for blue or purple flowers. Here we report on the isolation of a cDNA clone of a putative flavonoid-3',5'-hydroxylase gene from Phalaenopsis that was then cloned into a plant expression vector. Transient transformation was achieved by particle bombardment of Phalaenopsis petals. The transgenic petals changed from pink to magenta, indicating that the product of the putative flavonoid-3',5'-hydroxylase gene influences anthocyanin pigment synthesis.
花青素决定了花朵从红色到蓝色的颜色。蓝色和紫色花朵通常含有飞燕草素的衍生物，而红色和粉色花朵含有花青素或天竺葵素的衍生物。这三类主要花青素的羟基化模式差异由细胞色素P450酶控制。黄酮类-3',5'-羟化酶是细胞色素P450家族的成员，是合成3',5'-羟基化花青素的关键酶，通常是蓝色或紫色花朵所必需的。在这里，我们报告了从蝴蝶兰中分离出一个假定的黄酮类-3',5'-羟化酶基因的cDNA克隆，然后将其克隆到植物表达载体中。通过对蝴蝶兰花瓣进行粒子轰击实现了瞬时转化。转基因花瓣从粉色变为品红色，表明假定的黄酮类-3',5'-羟化酶基因的产物影响花青素色素的合成。
