Wang Joseph, Chen Gang, Chatrathi Madhu Prakash, Musameh Mustafa
Department of Chemistry and Biochemistry, New Mexico State University, Las Cruces, New Mexico 88003, USA.
Anal Chem. 2004 Jan 15;76(2):298-302. doi: 10.1021/ac035130f.
Significant improvements in the performance of a capillary electrophoresis (CE) microchip with an electrochemical detector are observed using a carbon nanotube (CNT)-modified working electrode. The CNT-modified electrode allows CE amperometric detection at significantly lower operating potentials and yields substantially enhanced signal-to-noise characteristics. The electrocatalytic detection is coupled to resistance to surface fouling and hence enhanced stability. Such advantages are illustrated in connection with several classes of hydrazine, phenol, purine, and amino acid compounds. Substantial minimization of surface fouling effects has been demonstrated in connection with the monitoring of phenol and tyrosine. Factors affecting the performance of the new CNT detector were assessed and optimized. CNTs from different sources are evaluated, and the effect of an anodic pretreatment is explored. The broad and significant catalytic activity exhibited by CNT-based CE detectors indicates great promise for a wide range of bioanalytical and environmental applications.
使用碳纳米管（CNT）修饰的工作电极时，可观察到配备电化学检测器的毛细管电泳（CE）微芯片性能有显著提升。CNT修饰电极可在显著更低的工作电位下进行CE安培检测，并产生大幅增强的信噪比特性。这种电催化检测与表面污垢抗性相关联，从而提高了稳定性。这些优势在几类肼、苯酚、嘌呤和氨基酸化合物的检测中得到了体现。在苯酚和酪氨酸的监测中，已证明表面污垢效应大幅降低。评估并优化了影响新型CNT检测器性能的因素。对不同来源的CNT进行了评估，并探讨了阳极预处理的效果。基于CNT的CE检测器所展现出的广泛且显著的催化活性表明，其在广泛的生物分析和环境应用中具有巨大潜力。
