Lisowski Piotr, Zarzycki Paweł K
Section of Toxicology and Bioanalytics, Koszalin University of Technology, Śniadeckich 2, 75-453 Koszalin, Poland.
Chromatographia. 2013;76(19):1201-1214. doi: 10.1007/s10337-013-2413-y. Epub 2013 Feb 22.
Microfluidic paper-based analytical devices and micro total analysis systems are relatively new group of analytical tools, capable of analyzing complex biochemical samples containing macromolecules, proteins, nucleic acids, toxins, cells or pathogens. Within one analytical run, fluidic manipulations like transportation, sorting, mixing or separation are available. Recently, microfluidic devices are a subject of extensive research, mostly for fast and non-expensive biochemical analysis but also for screening of medical samples and forensic diagnostics. They are used for neurotransmitter detection, cancer diagnosis and treatment, cell and tissue culture growth and amplification, drug discovery and determination, detection and identification of microorganisms. This review summarizes development history, basic fabrication methods, applications and also future development trends for production of such devices.
基于微流控纸的分析装置和微全分析系统是相对较新的一类分析工具，能够分析含有大分子、蛋白质、核酸、毒素、细胞或病原体的复杂生化样品。在一次分析运行中，可以进行诸如运输、分选、混合或分离等流体操作。最近，微流控装置是广泛研究的对象，主要用于快速且低成本的生化分析，也用于医学样本筛查和法医诊断。它们用于神经递质检测、癌症诊断与治疗、细胞和组织培养生长与扩增、药物发现与测定、微生物检测与鉴定。本文综述了此类装置的发展历史、基本制造方法、应用以及未来的发展趋势。
