Rusin Oleksandr, St Luce Nadia N, Agbaria Rezik A, Escobedo Jorge O, Jiang Shan, Warner Isiah M, Dawan Fareed B, Lian Kun, Strongin Robert M
Department of Chemistry and Center for Advanced Microstructures and Devices, Louisiana State University, Baton Rouge, Louisiana 70803, USA.
J Am Chem Soc. 2004 Jan 21;126(2):438-9. doi: 10.1021/ja036297t.
The determination of cysteine and homocysteine levels is of great current interest for the monitoring of desease states. A new colorimetric method for the simultaneous detection of l-cysteine and l-homocysteine has been developed. A fluorescein derivative reacts with the above amino acids, producing their respective thiazolidines resulting in color changes. Interference from other amino acids and proteins is minimal.
目前，测定半胱氨酸和高半胱氨酸水平对于监测疾病状态具有重要意义。已开发出一种用于同时检测L-半胱氨酸和L-高半胱氨酸的新比色法。一种荧光素衍生物与上述氨基酸发生反应，生成各自的噻唑烷，从而导致颜色变化。其他氨基酸和蛋白质的干扰极小。
