Xie Min-Qiang, Huang Yu-Lin, Xiao Jian-Yun, Zhao Su-Ping, Chen Zhao-Hui, Wang Cheng-Long
Department of Otolaryngology, The Third Affiliated Hospital, Sun Yat Sen University, Guangzhou, Guangdong, 510630, P.R.China.
Ai Zheng. 2004 Jan;23(1):104-7.
BACKGROUND & OBJECTIVE: The chromosomal aberration is common in nasopharyngeal carcinoma (NPC). The currently-used methods for detecting chromosomal abnormalities are complicated and of limited clinical value. The purpose of the present study was to explore the feasibility of detecting chromosomal abnormalities in NPC by rapid primed in situ labeling (RPRINS).
Using RPRINS technique with specific oligonucleotide primers of chromosome 3 and 7, the abnormalities of chromosome 3 and 7 in the frozen section tissues of 15 cases of NPC and 5 cases of normal nasopharyngeal mucosa (NNM) were detected. Loss of chromosome was defined when the percentage of cells with labeling signals </=1 was >/=65%, and the increase in chromosomal copy number was defined when the percentage of cells with labeling signals >/=3 was >/=6.5%.
In 15 cases of NPC tissues, the chromosome 3 had a labeling rate of 88.6% and increasing copy numbers in ten cases (66.7%), and the chromosome 7 had a labeling rate of 87.4% and loss of chromosome in five cases (33.3%). Four cases coexisted with increasing chromosome 3 copy numbers and loss of chromosome 7. In contrast, the labeling rates of chromosome 3 and 7 in NNM were 92.0% and 91.8%, respectively, and the percentage of diploid cells were 43.2% and 43.6%, respectively, with absence of triploid. There was a significant difference in the percentage of diploid cells between NPC and NNM (P< 0.05).
The technique of rapid PRINS could be used to detect chromosomes in frozen section tissues, and the chromosomal abnormalities would be helpful in diagnosis of NPC.
染色体畸变在鼻咽癌（NPC）中很常见。目前用于检测染色体异常的方法复杂且临床价值有限。本研究旨在探讨快速引物原位标记（RPRINS）检测NPC染色体异常的可行性。
采用针对3号和7号染色体的特异性寡核苷酸引物的RPRINS技术，检测15例NPC冰冻切片组织和5例正常鼻咽黏膜（NNM）中3号和7号染色体的异常情况。当标记信号≤1的细胞百分比≥65%时定义为染色体缺失，当标记信号≥3的细胞百分比≥6.5%时定义为染色体拷贝数增加。
在15例NPC组织中，3号染色体标记率为88.6%，10例（66.7%）存在拷贝数增加；7号染色体标记率为87.4%，5例（33.3%）存在染色体缺失。4例同时存在3号染色体拷贝数增加和7号染色体缺失。相比之下，NNM中3号和7号染色体的标记率分别为92.0%和91.8%，二倍体细胞百分比分别为43.2%和43.6%，无三倍体细胞。NPC和NNM中二倍体细胞百分比有显著差异（P<0.05）。
快速PRINS技术可用于检测冰冻切片组织中的染色体，染色体异常有助于NPC的诊断。
