Poore K R, Fowden A L
Department of Physiology, University of Cambridge, Cambridge, UK.
Diabetologia. 2004 Feb;47(2):340-8. doi: 10.1007/s00125-003-1305-3. Epub 2004 Jan 14.
AIMS/HYPOTHESIS: We have previously demonstrated poor glucose tolerance in adult pigs of naturally occurring low birthweight. The aim of this study was to examine sensitivity to insulin in juvenile (3-month-old) and adult (12-month-old) pigs of low and high birthweight.
Low (<1.47 kg) and high (>1.53 kg) birthweight piglets from 15 litters were studied at 3 (n=47) and 12 (n=17) months of age. At each age the selected pigs were tranquilised and catheters were inserted into the dorsal aorta and caudal vena cava under general anaesthesia. After recovery, insulin sensitivity was measured as the glucose decrement (mmol.l(-1).min(-1)) during the first 10 min after an intravenous insulin bolus (0.5 IU/kg). Data (means +/- SEM) were analysed by the Student's t test, ANOVA and linear regression.
The body weight of low birthweight female, but not male, pigs remained smaller than that of high birthweight pigs at 3 and 12 months of age. At 3 months, thinness at birth and rapid catch-up growth in the first month of life were associated with increased insulin sensitivity in males. In females thinness at 3 months was associated with reduced sensitivity to insulin. At 12 months, early postnatal catch-up growth was associated with insulin resistance, irrespective of sex, when all data were combined.
CONCLUSIONS/INTERPRETATION: The glucose intolerance previously observed in young adult pigs of low birthweight is probably due to insulin resistance. Early catch-up growth in low birthweight pigs was the clearest predictor of adult insulin resistance.
目的/假设：我们之前已经证明自然出生体重低的成年猪存在糖耐量受损的情况。本研究的目的是检测低出生体重和高出生体重的幼年（3月龄）及成年（12月龄）猪对胰岛素的敏感性。
从15窝仔猪中选取出生体重低（<1.47千克）和高（>1.53千克）的仔猪，分别在3月龄（n = 47）和12月龄（n = 17）时进行研究。在每个年龄段，将选定的猪麻醉后，在全身麻醉下将导管插入背主动脉和尾腔静脉。恢复后，静脉注射胰岛素推注（0.5 IU/kg）后，在最初10分钟内测量胰岛素敏感性，以葡萄糖下降量（mmol·l⁻¹·min⁻¹）表示。数据（均值±标准误）采用学生t检验、方差分析和线性回归进行分析。
低出生体重的雌性猪（而非雄性猪）在3月龄和12月龄时体重仍低于高出生体重的猪。在3月龄时，出生时体重较轻且在出生后第一个月快速追赶生长与雄性猪胰岛素敏感性增加有关。在雌性猪中，3月龄时体重较轻与胰岛素敏感性降低有关。在12月龄时，将所有数据合并后，无论性别如何，出生后早期追赶生长都与胰岛素抵抗有关。
结论/解读：之前在低出生体重的年轻成年猪中观察到的糖耐量受损可能是由于胰岛素抵抗。低出生体重猪的早期追赶生长是成年胰岛素抵抗最明显的预测指标。
