Hong H-S, Chung W H, Hung S I, Chen M J, Lee S H, Yang L C
Department of Dermatology, Chang Gung Memorial Hospital, Taipei, Taiwan.
Scand J Immunol. 2004 Jan;59(1):79-87. doi: 10.1111/j.0300-9475.2004.01353.x.
Anti-Golgi autoantibodies (AGAs) and their targets have been reported from several diseases. However, the association of AGAs, selective autoantigens and related clinical diseases is still obscure. In this study, the presence of AGAs in the sera of 5983 patients was screened to explore the association of AGAs and clinical diseases. By means of indirect immunofluorescence using HEp-2 cells, sera of 12 patients bearing AGAs were identified. The location of recognized Golgi autoantigen(s) was confirmed by the treatment of monensin and double immunostaining using beta-COP. Using the immunoelectron microscopy, AGA immunoreactivity was clearly demonstrated at a stack structure, characteristic of the Golgi complex. Furthermore, analysis of the 12 AGA-positive sera by Western blot revealed at least 15 components of Golgi antigens with relative molecular weights ranging from 54 to 350 kDa, and several Golgi autoantigens identified may be novel. Notably, over half of the AGA-positive cases found belong to non-autoimmune diseases, particularly hepatic disorder. This study presents the association of AGAs, components of the Golgi complex and clinical diseases.
抗高尔基体自身抗体（AGAs）及其靶抗原已在多种疾病中被报道。然而，AGAs、选择性自身抗原与相关临床疾病之间的关联仍不明确。在本研究中，对5983例患者血清中AGAs的存在情况进行了筛查，以探讨AGAs与临床疾病的关联。通过使用HEp-2细胞的间接免疫荧光法，鉴定出12例携带AGAs的患者血清。通过莫能菌素处理和使用β-COP的双重免疫染色，确认了被识别的高尔基体自身抗原的定位。利用免疫电子显微镜，在高尔基体复合体特有的堆叠结构处清楚地显示出AGAs免疫反应性。此外，通过蛋白质印迹法对12份AGAs阳性血清进行分析，发现至少有15种高尔基体抗原成分，其相对分子质量范围为54至350 kDa，并且鉴定出的几种高尔基体自身抗原可能是新的。值得注意的是，发现的AGAs阳性病例中超过一半属于非自身免疫性疾病，尤其是肝脏疾病。本研究揭示了AGAs、高尔基体复合体成分与临床疾病之间的关联。
