拟南芥不同种质间的基因组大小变异
Genome size variation among accessions of Arabidopsis thaliana.
作者信息
Schmuths Heike, Meister Armin, Horres Ralf, Bachmann Konrad
机构信息
Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstr. 3, D-06466 Gatersleben, Germany.
出版信息
Ann Bot. 2004 Mar;93(3):317-21. doi: 10.1093/aob/mch037. Epub 2004 Jan 14.
Abstract
BACKGROUND AND AIMS
Estimates of the amount of nuclear DNA of Arabidopsis thaliana, known to be among the lowest within angiosperms, vary considerably. This study aimed to determine genome size of a range of accessions from throughout the entire Eurasian range of the species.
METHODS
Twenty accessions from all over Europe and one from Japan were examined using flow cytometry.
KEY RESULTS
Significant differences in mean C-values were detected over a 1.1-fold range. Mean haploid (1C) genome size was 0.215 pg (211 Mbp) for all analysed accessions. Two accessions were tetraploid.
CONCLUSIONS
A closer investigation of the DNA fractions involved in intraspecific genome size differences in this experimentally accessible species may provide information on the factors involved in stability and evolution of genome sizes.
摘要
背景与目的
拟南芥的核DNA含量估计在被子植物中处于最低水平，但其估计值差异很大。本研究旨在确定该物种在整个欧亚分布范围内一系列种质的基因组大小。
方法
使用流式细胞术对来自欧洲各地的20种种质和来自日本的1种种质进行了检测。
主要结果
在1.1倍的范围内检测到平均C值存在显著差异。所有分析种质的单倍体（1C）基因组平均大小为0.215皮克（211兆碱基对）。有两个种质是四倍体。
结论
对这个实验上易于研究的物种种内基因组大小差异所涉及的DNA组分进行更深入的研究，可能会提供有关基因组大小稳定性和进化所涉及因素的信息。
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