Kapo Naida, Omeragić Jasmin, Bačić Azra, Goletić Šejla, Softić Adis, Škapur Vedad, Eterović Toni, Salkić Jasna, Goletić Teufik
University of Sarajevo - Veterinary Faculty, Sarajevo, Bosnia and Herzegovina.
Institute for Biomedical Diagnostics and Research - GENOM, Travnik, Bosnia and Herzegovina.
Int J Parasitol Parasites Wildl. 2025 Jun 11;27:101099. doi: 10.1016/j.ijppaw.2025.101099. eCollection 2025 Aug.
, the causative agent of alveolar echinococcosis (AE), is a parasitic zoonosis of increasing public health significance in Europe. Although previously confirmed in red foxes in Bosnia and Herzegovina, this study provides the first report of in golden jackals () in the country. Between December 2024 and March 2025, a total of 44 golden jackals were examined across 15 localities in Bosnia and Herzegovina, with detected in 6.8 % of the samples from three sites in Western and Central Bosnia and Herzegovina. Adult spp. worms were first detected using the intestinal scraping technique and identified by microscopy. To confirm these findings and differentiate between species, DNA extracted from adult worms was then subjected to species-specific PCR targeting a fragment of the mitochondrial 12S ribosomal RNA gene. PCR-positive samples for were further validated by next-generation sequencing (NGS) of a 203 bp amplicon of the 12S rRNA gene. These findings indicate an expanding distribution of in Bosnia and Herzegovina, highlighting the role of golden jackals as definitive hosts for the parasite. Given the growing jackal population in the country and the increasing public health concerns, enhanced surveillance and further research are warranted, particularly regarding human cases of infection, to better understand the associated epidemiological risks.
泡型包虫病（AE）的病原体，是一种在欧洲公共卫生意义日益增加的寄生虫人畜共患病。尽管此前在波斯尼亚和黑塞哥维那的赤狐中得到证实，但本研究首次报告了该国金豺（）体内存在该病原体。在2024年12月至2025年3月期间，在波斯尼亚和黑塞哥维那的15个地点共检查了44只金豺，在波斯尼亚和黑塞哥维那西部和中部三个地点的6.8%的样本中检测到该病原体。首先使用肠道刮取技术检测到成年棘球绦虫属蠕虫，并通过显微镜鉴定。为了证实这些发现并区分棘球绦虫属物种，然后对从成年蠕虫中提取的DNA进行针对线粒体12S核糖体RNA基因片段的物种特异性PCR。通过对12S rRNA基因的203 bp扩增子进行下一代测序（NGS）进一步验证了棘球绦虫属的PCR阳性样本。这些发现表明棘球绦虫属在波斯尼亚和黑塞哥维那的分布正在扩大，突出了金豺作为该寄生虫终末宿主的作用。鉴于该国豺狼数量的增加以及日益增加的公共卫生问题，有必要加强监测和进一步研究，特别是关于人类棘球绦虫属感染病例，以更好地了解相关的流行病学风险。
