Omeragić Jasmin, Goletić Teufik, Softić Adis, Goletić Šejla, Kapo Naida, Soldo Darinka Klarić, Šupić Jovana, Škapur Vedad, Čerkez Goran, Ademović Enisa, Semren Orjana, Alić Amer
University of Sarajevo - Veterinary Faculty, Sarajevo, Bosnia and Herzegovina.
University of Sarajevo - Faculty of Agriculture and Food Science, Sarajevo, Bosnia and Herzegovina.
Int J Parasitol Parasites Wildl. 2022 Nov 12;19:269-272. doi: 10.1016/j.ijppaw.2022.11.005. eCollection 2022 Dec.
has been spreading through Central Eastern Europe but has not yet been reported in Bosnia and Herzegovina (B&H). Recently, this parasite is confirmed in Croatia suggesting the movement of the parasite's distribution limit further south. Given that there is no surveillance or monitoring system for echinococcosis in B&H, our study was designed as a pilot study of . A total of 57 red foxes originating from 24 localities all over the country were collected during the routine rabies monitoring, autopsied and examined for the presence of echinococcosis. Based on intestinal scraping technique and microscopy, adult worms have been detected in one (1/57, 1.75%) red fox. To verify this finding and to differentiate spp., DNA extracted from adult worms was subjected to species-specific PCR targeting part of the mitochondrial 12S ribosomal RNA gene. PCR-positive samples were further confirmed by NGS sequencing of a 203 bp amplified fragment of 12S rRNA, which has been deposited in GenBank (Accession no.: OP047920). This finding represents the first detection of in B&H, strongly suggesting its presence in the country. The confirmation of the parasite in the same locality where migrants/refugees temporarily stay on their route to Western Europe highlights the need for a One Health approach in addressing all future questions. Moreover, the first detection of in B&H warrants the need for the implementation of an appropriate state surveillance program.
已在中东欧传播，但波斯尼亚和黑塞哥维那（波黑）尚未有相关报告。最近，这种寄生虫在克罗地亚得到确认，这表明其分布界限进一步向南移动。鉴于波黑没有棘球蚴病监测系统，我们的研究被设计为一项关于……的试点研究。在常规狂犬病监测期间，共收集了来自该国各地24个地点的57只赤狐，进行解剖并检查是否存在棘球蚴病。基于肠道刮片技术和显微镜检查，在一只（1/57，1.75%）赤狐中检测到成虫。为验证这一发现并区分……物种，从成虫中提取的DNA进行了针对线粒体12S核糖体RNA基因部分的物种特异性PCR。PCR阳性样本通过对12S rRNA的203 bp扩增片段进行NGS测序进一步确认，该片段已存入GenBank（登录号：OP047920）。这一发现代表了在波黑首次检测到……，强烈表明其在该国存在。在移民/难民前往西欧途中临时停留的同一地点确认这种寄生虫，凸显了采用“同一健康”方法解决所有未来问题的必要性。此外，在波黑首次检测到……，有必要实施适当的国家监测计划。
