Zhang Hong-Ye, Lu Jin-Chun
Clinical Laboratory, Nanjing Yuhua Hospital, Nanjing, Jiangsu 210012, China.
Zhonghua Nan Ke Xue. 2003 Dec;9(9):687-9.
To obtain Chlamydia trachomatis heat shock protein (cHSP) 10 gene from clinical secretion samples.
cHSP10 gene was amplified from 20 cases of clinical secretion samples with positive gold-labelling by specific primers of cHSP10 and identified by sequence analysis.
cHSP10 full-length gene was amplified from 1 of 20 cases of clinical secretion samples with positive gold-labelling. cHSP10 gene encoding 102 amino acids contains 306 bp, which nuclotide at position 194 changes from T to A, leading to the change of corresponding amino acid.
cHSP10 gene may be cloned from clinical secretion samples with positive gold-labelling, which make it possible to further construct expression plasmid of recombinant cHSP10.
从临床分泌物样本中获取沙眼衣原体热休克蛋白（cHSP）10基因。
采用cHSP10特异性引物，从20例金标阳性的临床分泌物样本中扩增cHSP10基因，并进行序列分析鉴定。
从20例金标阳性的临床分泌物样本中的1例扩增出cHSP10全长基因。cHSP10基因编码102个氨基酸，含306 bp，其中第194位核苷酸由T变为A，导致相应氨基酸改变。
可从金标阳性的临床分泌物样本中克隆cHSP10基因，这为进一步构建重组cHSP10表达质粒奠定了基础。
