[Cloning and sequence analysis of Chlamydia trachomatis heat shock protein 10].

OBJECTIVE

To obtain Chlamydia trachomatis heat shock protein (cHSP) 10 gene from clinical secretion samples.

METHODS

cHSP10 gene was amplified from 20 cases of clinical secretion samples with positive gold-labelling by specific primers of cHSP10 and identified by sequence analysis.

RESULTS

cHSP10 full-length gene was amplified from 1 of 20 cases of clinical secretion samples with positive gold-labelling. cHSP10 gene encoding 102 amino acids contains 306 bp, which nuclotide at position 194 changes from T to A, leading to the change of corresponding amino acid.

CONCLUSIONS

cHSP10 gene may be cloned from clinical secretion samples with positive gold-labelling, which make it possible to further construct expression plasmid of recombinant cHSP10.