乙醇对肺静脉心肌细胞电生理特性的影响。

Effect of ethanol on the electrophysiological characteristics of pulmonary vein cardiomyocytes.

作者信息

Chen Yao-Chang, Chen Shih-Ann, Chen Yi-Jen, Tai Ching-Tai, Chan Paul, Lin Cheng-I

机构信息

Department of Biomedical Engineering, National Defense Medical Center, Taipei, Taiwan

出版信息

Eur J Pharmacol. 2004 Jan 12;483(2-3):215-22. doi: 10.1016/j.ejphar.2003.10.042.

DOI:10.1016/j.ejphar.2003.10.042

PMID:14729109

Abstract

Ethanol consumption has been considered to contribute to the occurrences of paroxysmal atrial fibrillation. Pulmonary veins are known to initiate atrial fibrillation. This study investigated whether ethanol may induce atrial fibrillation through increasing arrhythmogenic activity of pulmonary vein cardiomyocytes. Using the whole-cell clamp technique, the action potential and ionic currents were investigated in rabbit single pulmonary vein beating cardiomyocytes with and without (control) incubation of ethanol. Compared with control cardiomyocytes, pulmonary vein cardiomyocytes receiving 0.3 mg/ml or 1 mg/ml ethanol had shorter action potential duration, but had similar beating rates (2.6+/-1.3, 2.7+/-1.2, 2.7+/-1.2 Hz) and incidences (45%, 41%, 32%) of delayed after depolarization. Pulmonary vein cardiomyocytes receiving ethanol had smaller L-type Ca(2+) currents and larger transient outward currents, but had similar transient inward, delayed rectified outward, inward rectified and pacemaker currents. These results suggest that ethanol has no direct effect on the arrhythmogenic potential of pulmonary vein cardiomyocytes.

摘要

饮酒被认为与阵发性心房颤动的发生有关。已知肺静脉会引发心房颤动。本研究调查了乙醇是否可能通过增加肺静脉心肌细胞的致心律失常活性来诱发心房颤动。采用全细胞膜片钳技术，在有和没有（对照）乙醇孵育的兔单个肺静脉跳动心肌细胞中研究动作电位和离子电流。与对照心肌细胞相比，接受0.3mg/ml或1mg/ml乙醇的肺静脉心肌细胞动作电位时程较短，但搏动频率相似（2.6±1.3、2.7±1.2、2.7±1.2Hz），延迟后去极化发生率相似（45%、41%、32%）。接受乙醇的肺静脉心肌细胞L型钙电流较小，瞬时外向电流较大，但瞬时内向、延迟整流外向、内向整流和起搏电流相似。这些结果表明，乙醇对肺静脉心肌细胞的致心律失常潜能没有直接影响。