Karabiber Hamza, Yakinci Cengiz, Durmaz Yasar, Temel Ismail, Mehmet Nihayet
KSU Medical School, Department of Pediatrics, 46050, Kahramanmaras, Turkey.
Brain Dev. 2004 Jan;26(1):15-8. doi: 10.1016/s0387-7604(03)00076-7.
In experimental epilepsy studies, nitric oxide was found to act as both proconvulsant and anticonvulsant. The objective of this study was to investigate the effects of valproic acid and carbamazepine on serum levels of nitrite and nitrate, which are the metabolites of nitric oxide. To achieve this goal, serum nitrite and nitrate levels were determined in active epileptic 34 children using valproic acid and 23 children using carbamazepine and in non-active epileptic 38 children (control group) not using any antiepileptic drug. In the valproic acid group serum nitrite and nitrate levels were 2.66 +/- 2.11 micromol/l and 69.35 +/- 23.20 micromol/l, 1.89 +/- 1.01 micromol/l and 49.39 +/- 10.61 micromol/l in the carbamazepine group, and 1.22 +/- 0.55 micromol/l, 29.53 +/- 10.05 micromol in the control group, respectively. Nitrite and nitrate levels were significantly high in both valproic acid and carbamazepine groups compared to the control group (P < 0.01). When valproic acid and carbamazepine groups were compared to each other, level of nitrate was found statistically higher in the valproic acid group in relation to the carbamazepine group (P < 0.01), however, there was no statistically significant difference in the levels of nitrite (P > 0.05). No relation could be found between serum drug levels and nitrite and nitrate levels. According to these results, it can be suggested that valproic acid and carbamazepine might have antiepileptic effects through nitric oxide.
在实验性癫痫研究中，发现一氧化氮兼具促惊厥和抗惊厥作用。本研究的目的是调查丙戊酸和卡马西平对作为一氧化氮代谢产物的血清亚硝酸盐和硝酸盐水平的影响。为实现这一目标，测定了34名使用丙戊酸的活动性癫痫儿童、23名使用卡马西平的活动性癫痫儿童以及38名未使用任何抗癫痫药物的非活动性癫痫儿童（对照组）的血清亚硝酸盐和硝酸盐水平。丙戊酸组血清亚硝酸盐和硝酸盐水平分别为2.66±2.11微摩尔/升和69.35±23.20微摩尔/升，卡马西平组为1.89±1.01微摩尔/升和49.39±10.61微摩尔/升，对照组为1.22±0.55微摩尔/升、29.53±10.05微摩尔/升。与对照组相比，丙戊酸组和卡马西平组亚硝酸盐和硝酸盐水平均显著升高（P<0.01）。丙戊酸组和卡马西平组相互比较时，发现丙戊酸组硝酸盐水平相对于卡马西平组在统计学上更高（P<0.01），然而，亚硝酸盐水平无统计学显著差异（P>0.05）。未发现血清药物水平与亚硝酸盐和硝酸盐水平之间存在关联。根据这些结果，可以推测丙戊酸和卡马西平可能通过一氧化氮发挥抗癫痫作用。
