Nie Shuping, Tian Baoyu, Wang Xiaowei, Pincus David H, Welker Martin, Gilhuley Kathleen, Lu Xuedong, Han Yiping W, Tang Yi-Wei
Department of Laboratory Medicine, Futian Hospital, Guangdong Medical College, Shenzhen, China Department of Laboratory Medicine, Memorial Sloan-Kettering Cancer Center, New York, New York, USA.
Department of Periodontics, Case Western Reserve University, Cleveland, Ohio, USA.
J Clin Microbiol. 2015 Apr;53(4):1399-402. doi: 10.1128/JCM.00239-15. Epub 2015 Feb 4.
We explored the use of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for identification of Fusobacterium nucleatum subspecies. MALDI-TOF MS spectra of five F. nucleatum subspecies (animalis, fusiforme, nucleatum, polymorphum, and vincentii) were analyzed and divided into four distinct clusters, including subsp. animalis, nucleatum, polymorphum, and fusiforme/vincentii. MALDI-TOF MS with the modified SARAMIS database further correctly identified 28 of 34 F. nucleatum clinical isolates to the subspecies level.
我们探索了使用基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)来鉴定具核梭杆菌亚种。分析了五个具核梭杆菌亚种(动物亚种、梭形亚种、具核亚种、多形亚种和文森亚种)的MALDI-TOF MS谱图,并将其分为四个不同的簇,包括动物亚种、具核亚种、多形亚种以及梭形/文森亚种。使用经过修改的SARAMIS数据库的MALDI-TOF MS进一步将34株具核梭杆菌临床分离株中的28株准确鉴定到亚种水平。