Centre for Chemistry and Biotechnology, Deakin University, Geelong, VIC, 3216, Australia.
J Ind Microbiol Biotechnol. 2013 Nov;40(11):1231-40. doi: 10.1007/s10295-013-1324-0. Epub 2013 Aug 30.
Marine heterotrophic microbes are capable of accumulating large amounts of lipids, omega-3 fatty acids, carotenoids, and have potential for biodiesel production. Pollen baiting using Pinus radiata pollen grain along with direct plating techniques were used in this study as techniques for the isolation of oil-producing marine thraustochytrid species from Queenscliff, Victoria, Australia. Thirteen isolates were obtained using either direct plating or using pine pollen, with pine pollen acting as a specific substrate for the surface attachment of thraustochytrids. The isolates obtained from the pollen baiting technique showed a wide range of docosahexaenoic acid (DHA) accumulation, from 11 to 41 % of total fatty acid content (TFA). Direct plating isolates showed a moderate range of DHA accumulation, from 19 to 25 % of TFA. Seven isolates were identified on the basis of 18S rRNA sequencing technique as Thraustochytrium species, Schizochytrium species, and Ulkenia species. Although both methods appear to result in the isolation of similar strains, pollen baiting proved to be a simpler method for the isolation of these relatively slow-growing organisms.
海洋异养微生物能够大量积累油脂、ω-3 脂肪酸、类胡萝卜素，并且具有生产生物柴油的潜力。本研究采用辐射松花粉粒和直接平板技术作为从澳大利亚维多利亚州昆斯克利夫分离产油海洋硫球藻属物种的方法。使用直接平板或松花粉获得了 13 个分离株，松花粉作为硫球藻属物种表面附着的特定底物。从花粉诱饵技术获得的分离株显示出 DHA 积累的广泛范围，从总脂肪酸含量 (TFA) 的 11%到 41%。直接平板分离株显示出中等范围的 DHA 积累，从 TFA 的 19%到 25%。基于 18S rRNA 测序技术，有 7 个分离株被鉴定为硫球藻属、裂殖壶菌属和 Ulkenia 属。尽管这两种方法似乎都能分离出类似的菌株，但花粉诱饵法被证明是一种更简单的方法，可以分离出这些生长相对较慢的生物。
