Riemenschneider Markus J, Koy Timmo H, Reifenberger Guido
Department of Neuropathology, Heinrich-Heine-University, Moorenstrasse 5, 40225 Duesseldorf, Germany.
Acta Neuropathol. 2004 Mar;107(3):277-82. doi: 10.1007/s00401-003-0809-8. Epub 2004 Jan 17.
The oligodendrocyte lineage genes OLIG1 and OLIG2 have been reported as potential diagnostic markers for oligodendrogliomas [Lu et al. (2001) Proc Natl Acad Sci USA 98:10851-10856; Marie et al. (2001) Lancet 358:298-300]. We investigated the mRNA expression of OLIG1 and OLIG2, as well as four other genes involved in oligodendrocyte development ( E2A, HEB, NKX2.2, and PDGFRA) in a panel of 70 gliomas, including 9 oligodendrogliomas, 11 anaplastic oligodendrogliomas, 5 oligoastrocytomas, 10 anaplastic oligoastrocytomas, 10 diffuse astrocytomas, 10 anaplastic astrocytomas, and 15 glioblastomas. Most tumors demonstrated higher transcript levels of these genes as compared to non-neoplastic adult brain tissue. Four glioblastomas showed markedly increased PDGFRA mRNA expression due to PDGFRA gene amplification. Statistical analyses revealed no significant expression differences between oligodendroglial and astrocytic tumors. In oligodendroglial tumors, expression of the six genes was not significantly correlated to loss of heterozygosity on chromosome arms 1p and 19q. Thus, expression of the investigated oligodendrocyte lineage genes is up-regulated relative to non-neoplastic brain tissue in the majority of oligodendroglial and astrocytic tumors, suggesting that glioma cells are arrested in or recapitulate molecular phenotypes corresponding to early stages of glial development. However, the determination of mRNA expression of these genes by means of reverse transcription-PCR does not appear to be diagnostically useful as a marker for oligodendrogliomas.
少突胶质细胞谱系基因OLIG1和OLIG2已被报道为少突胶质细胞瘤的潜在诊断标志物[Lu等人（2001年），《美国国家科学院院刊》98:10851 - 10856；Marie等人（2001年），《柳叶刀》358:298 - 300]。我们在一组70例胶质瘤中研究了OLIG1和OLIG2以及其他四个参与少突胶质细胞发育的基因（E2A、HEB、NKX2.2和PDGFRA）的mRNA表达，这些胶质瘤包括9例少突胶质细胞瘤、11例间变性少突胶质细胞瘤、5例少突星形细胞瘤、10例间变性少突星形细胞瘤、10例弥漫性星形细胞瘤、10例间变性星形细胞瘤和15例胶质母细胞瘤。与非肿瘤性成人大脑组织相比，大多数肿瘤显示出这些基因更高的转录水平。4例胶质母细胞瘤由于PDGFRA基因扩增而显示出明显增加的PDGFRA mRNA表达。统计分析显示少突胶质细胞肿瘤和星形细胞肿瘤之间没有显著的表达差异。在少突胶质细胞肿瘤中，这六个基因的表达与染色体臂1p和19q上的杂合性缺失没有显著相关性。因此，在大多数少突胶质细胞和星形细胞肿瘤中，所研究的少突胶质细胞谱系基因的表达相对于非肿瘤性脑组织上调，这表明胶质瘤细胞停滞在或重现了与神经胶质发育早期阶段相对应的分子表型。然而，通过逆转录 - PCR测定这些基因的mRNA表达似乎作为少突胶质细胞瘤的标志物在诊断上并无用处。
