Tong L J, Yamaguchi N, Kita M, Imanishi J
Department of Microbiology, Kyoto Prefectural University of Medicine, Japan.
Cell Struct Funct. 1992 Oct;17(5):257-61. doi: 10.1247/csf.17.257.
It is well known that interferons inhibit cell growth. However, we found that human interferon-gamma (HuIFN-gamma) enhanced the growth of human osteosarcoma cells, HOS-Y1 cells, in a dose-dependent manner. This enhancing effect was found only under the following conditions: when the cells were precultured for 2 or 3 days and then treated with HuIFN-gamma for 2, 3, or 4 days, and when the cells were seeded at a density of 1,000 or 2,000 cells/well. The degree of enhancement of cell growth was maximum when the cells were precultured at a density of 1,000 cells/well for 3 days and then treated with HuIFN-gamma for 2 days. The enhancing effect of HuIFN-gamma disappeared in the presence of anti-HuIFN-gamma antibody. In addition, it was found that the conditioned medium from HOS-Y1 cells enhanced the growth of HOS-Y1 cells, and that the conditioned medium from HOS-Y1 cells cultured with HuIFN-gamma enhanced the cell growth more than that from cells cultured without HuIFN-gamma. Epidermal growth factor (EGF), acidic fibroblast growth factor (aFGF), basic fibroblast growth factor (bFGF), and transforming growth factor-beta 1(TGF-beta 1) did not enhance the growth of HOS-Y1 cells. These results suggest that HuIFN-gamma enhanced the cell growth by augmenting the production of unknown growth factor(s) in HOS-Y1 cells via an autocrine mechanism.
众所周知，干扰素会抑制细胞生长。然而，我们发现人γ干扰素（HuIFN-γ）能以剂量依赖的方式促进人骨肉瘤细胞HOS-Y1细胞的生长。仅在以下条件下发现这种促进作用：当细胞预培养2或3天，然后用HuIFN-γ处理2、3或4天，以及当细胞以1000或2000个细胞/孔的密度接种时。当细胞以1000个细胞/孔的密度预培养3天，然后用HuIFN-γ处理2天时，细胞生长的促进程度最大。在抗HuIFN-γ抗体存在的情况下，HuIFN-γ的促进作用消失。此外，发现来自HOS-Y1细胞的条件培养基能促进HOS-Y1细胞的生长，并且与未用HuIFN-γ培养的细胞相比，用HuIFN-γ培养的HOS-Y1细胞的条件培养基对细胞生长的促进作用更大。表皮生长因子（EGF）、酸性成纤维细胞生长因子（aFGF）、碱性成纤维细胞生长因子（bFGF）和转化生长因子-β1（TGF-β1）均未促进HOS-Y1细胞的生长。这些结果表明，HuIFN-γ通过自分泌机制增加HOS-Y1细胞中未知生长因子的产生来促进细胞生长。
