de Melo E J, de Carvalho T U, de Souza W
Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Ilha do Fundäo, Brasil.
Cell Struct Funct. 1992 Oct;17(5):311-7. doi: 10.1247/csf.17.311.
Fluorescence microscopy, using dyes which specifically label mitochondria, endoplasmic reticulum and the Golgi complex, and transmission electron microscopy, were used to analyze the changes which occur in the organization of these structures during interaction of Toxoplasma gondii with host cells. In uninfected cells the mitochondria are long filamentous structures which radiate from the nuclear region toward the cell periphery. After parasite penetration they become shorter and tend to concentrate around the parasite-containing vacuole (parasitophorous vacuole) located in the cytoplasm of the host cell. The mitochondria of extracellular parasites, but not of those located within the parasitophorous vacuole, were also stained by rhodamine 123. Labeling with DiOC6, which binds to elements of the endoplasmic reticulum, in association with transmission electron microscopy, revealed a concentration of this structure around the parasitophorous vacuole. The membrane lining this vacuole was also stained, suggesting that components of the endoplasmic reticulum are also incorporated into this membrane. The Golgi complex, as revealed by staining with NBD-ceramide and electron microscopy, maintains its perinuclear position throughout the evolution of the intracellular parasitism.
利用能特异性标记线粒体、内质网和高尔基体复合体的染料进行荧光显微镜观察，以及透射电子显微镜观察，来分析在弓形虫与宿主细胞相互作用期间这些结构的组织变化。在未感染的细胞中，线粒体是长丝状结构，从核区向细胞周边辐射。寄生虫侵入后，它们会变短，并倾向于集中在位于宿主细胞质中的含寄生虫液泡（寄生泡）周围。细胞外寄生虫的线粒体，而非寄生泡内的寄生虫的线粒体，也被罗丹明123染色。与透射电子显微镜观察相结合，用与内质网成分结合的DiOC6标记显示，这种结构集中在寄生泡周围。衬于该液泡的膜也被染色，表明内质网的成分也被整合到该膜中。如用NBD - 神经酰胺染色和电子显微镜观察所显示的，高尔基体复合体在细胞内寄生的整个过程中都保持其核周位置。
