非酿酒酵母嗜腺嘌呤阿苏鲁酵母LS3线粒体DNA的基因组组织

Genome organization of mitochondrial DNA from the non-saccharomycete yeast Arxula adeninivorans LS3.

作者信息

Pich U, Kunze G

机构信息

Institute of Plant Genetics and Crop Plant Research, Gatersleben, Federal Republic of Germany.

出版信息

Curr Genet. 1992 Dec;22(6):505-6. doi: 10.1007/BF00326417.

DOI:10.1007/BF00326417

PMID:1473183

Abstract

Mitochondrial (mt) DNA of the asexual ascomycetous yeast Arxula adeninivorans LS3 was isolated and characterized. The mtDNA has a GC content of 30.3 mol%. It is circular and its size, as estimated by restriction analysis performed with nine endonucleases, was 35.5 kbp. Using mt gene-probes from Saccharomyces cerevisiae six structural genes (cob, cox1, cox2, oli1, oli2, and 21S rRNA) were located on the mitochondrial genome of A. adeninivorans. The comparison between the mt genomes of A. adeninivorans and other yeasts showed differences in genome organization.

摘要

对无性子囊菌酵母阿氏丝孢酵母LS3的线粒体（mt）DNA进行了分离和表征。该mtDNA的GC含量为30.3摩尔%。它是环状的，通过用9种核酸内切酶进行的限制性分析估计，其大小为35.5千碱基对。利用酿酒酵母的mt基因探针，在阿氏丝孢酵母的线粒体基因组上定位了6个结构基因（cob、cox1、cox2、oli1、oli2和21S rRNA）。阿氏丝孢酵母与其他酵母的mt基因组比较显示，基因组组织存在差异。