Gienow U, Kunze G, Schauer F, Bode R, Hofemeister J
Zentralinstitut für Genetik und Kulturpflanzenforschung der Akademie der Wissenschaften der DDR, Gatersleben.
Zentralbl Mikrobiol. 1990;145(1):3-12.
The genomic structure of an industrial yeast strain Trichosporon spec. LS3 was compared with Trichosporon adeninovorans type strain CBS 8244. The cot values, the portion of single copy sequences (CBS 8244: 10.9 x 10(9) D, LS3: 10.6 x 10(9) D) as well as of repetitive sequences (CBS 8244: 6.0 x 10(9) D, LS3: 5.5 x 10(9) D) per haploid genome and genome complexity of these strains (CBS 2844: 16.9 x 10(9) D, LS3: 16.1 x 10(9) D) have been analysed. Both strains show a high genome complexity. The mitochondrial DNA content was measured and compared. No plasmidal DNA was identified for both strains. A survey of the data of genomic DNA made it possible to postulate for each of both strains a haploid set of chromosomes. The intraspecific reassoziation values of the nuclear DNA from the strain LS3 and CBS 8244 are interpreted to confirm classification of these strains based on physiological and genetical properties into one species Trichosporon adeninovorans.
将工业酵母菌株Trichosporon spec. LS3的基因组结构与食腺Trichosporon的模式菌株CBS 8244进行了比较。分析了这两个菌株每个单倍体基因组的共t值、单拷贝序列部分（CBS 8244：10.9×10⁹ D，LS3：10.6×10⁹ D）以及重复序列部分（CBS 8244：6.0×10⁹ D，LS3：5.5×10⁹ D）和基因组复杂度（CBS 2844：16.9×10⁹ D，LS3：16.1×10⁹ D）。两个菌株均显示出较高的基因组复杂度。对线粒体DNA含量进行了测定和比较。未在两个菌株中鉴定出质粒DNA。对基因组DNA数据的调查使得能够为两个菌株中的每一个假定一个单倍体染色体组。菌株LS3和CBS 8244的核DNA种内重配值被解释为证实了基于生理和遗传特性将这些菌株分类为一个食腺Trichosporon物种。
