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Mapping of the glucoamylase gene of Trichosporon adeninovorans by mitotic haploidization using hybrids from protoplast fusions.

作者信息

Büttner R, Bode R, Samsonova I A, Birnbaum D

机构信息

Sektion Biologie der Ernst-Moritz-Arndt-Universität, Greifswald, DDR.

出版信息

J Basic Microbiol. 1990;30(4):227-31. doi: 10.1002/jobm.3620300402.

DOI:10.1002/jobm.3620300402
PMID:2117065
Abstract

In two strains of Trichosporon adeninovorans genes of unknown location were assigned to linkage groups. Diploid strains were produced by protoplast fusion and partially haploidized by growth on benomyl causing mitotic haploidization. We show that both strains synthesize an extracellular glucoamylase with different properties. These differences could be used to map the corresponding gene in the segregants. We could map the glucoamylase gene from one of these yeast strains on linkage group II, one of the three linkage groups identified so far.

摘要

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Mapping of the glucoamylase gene of Trichosporon adeninovorans by mitotic haploidization using hybrids from protoplast fusions.
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