Allouche Noureddine, Fki Ines, Sayadi Sami
Laboratoire des Bio-procédés, Centre de Biotechnologie de Sfax, B.P. K 3038, Sfax, Tunisia.
J Agric Food Chem. 2004 Jan 28;52(2):267-73. doi: 10.1021/jf034944u.
We investigated to develop effective procedures to recover the potentially high-added-value phenolic compounds contained in the discontinuous three-phase olive processing wastewaters (OMW). Particular emphasis was made to extract and purify hydroxytyrosol, one of the major compounds occurring in OMW. Batch optimization experiments showed that ethyl acetate is the most efficient solvent for the recovery of phenolic monomers from OMW. The latter was used with an optimal pH equal to 2. Furthermore, the percentage of each monomer, and particularly hydroxytyrosol, in the extract was maximum for a solvent ratio and a theoretical extraction stage number equal to 2 and 3, respectively. High yield (85.46%) recovery of hydroxytyrosol was achieved from OMW using a three-staged continuous counter-current liquid-liquid extraction unit. Hydroxytyrosol (1.225 g) were extracted per liter of OMW. One gram of hydroxytyrosol per liter of OMW was then purified by means of a chromatographic system which could be adapted to a large scale production process.
我们开展研究以开发有效的程序，用于回收间断三相橄榄加工废水中所含的具有潜在高附加值的酚类化合物。特别着重于提取和纯化羟基酪醇，它是橄榄加工废水中主要的化合物之一。间歇优化实验表明，乙酸乙酯是从橄榄加工废水中回收酚类单体最有效的溶剂。使用乙酸乙酯时的最佳pH值等于2。此外，对于溶剂比和理论萃取级数分别等于2和3的情况，提取物中每种单体（特别是羟基酪醇）的百分比最高。使用三级连续逆流液液萃取装置从橄榄加工废水中实现了羟基酪醇的高产率（85.46%）回收。每升橄榄加工废水可提取1.225克羟基酪醇。然后，每升橄榄加工废水中的1克羟基酪醇通过一种可适用于大规模生产过程的色谱系统进行纯化。
