Hamden Khaled, Allouche Noureddine, Damak Mohamed, Elfeki Abdelfattah
Animal Ecophysiology, Faculty of Sciences, Sfax, Tunisia.
Chem Biol Interact. 2009 Aug 14;180(3):421-32. doi: 10.1016/j.cbi.2009.04.002. Epub 2009 Apr 23.
This study aimed to evaluate the effect of phenolic extract and purified hydroxytyrosol (HT) from olive mill waste (OMW) on oxidative stress and hyperglycemia in alloxan-induced diabetic rats. The OMW biophenols were extracted using ethyl acetate. The obtained extract was fractionated by solid phase extraction (SPE) experimentation to generate two fractions: (F1) and (F2). HPLC-UV and HPLC-MS analysis showed that (F1) was made of known OMW monomeric phenolics mainly hydroxytyrosol (HT) while (F2) contained oligomeric and polymeric phenols such as verbascosid and ligstrosid. (HT) was purified from (F1) using silica gel-column chromatography and silica gel-TLC techniques. In incubated pancreas, supplementation of OMW fractions enhanced insulin secretion. The administration of OMW extract fractions (F1) and (F2) as well as purified (HT) in diabetic rats caused a decrease in glucose level in plasma and an increase in renal superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX) activities in liver and kidney. Furthermore, a protective action against hepatic and renal toxicity in diabetic rats was clearly observed. Furthermore, a significant decrease in hepatic and renal indices toxicity was observed, i.e. alkalines phosphatases (ALP), aspartate and lactate transaminases (AST and ALT) activities and the thiobarbituric acid-reactive substances (TBARs), total and direct bilirubin, creatinine and urea levels. In addition, (F1), (F2) and especially (HT) decreased triglycerides (TG), total-cholesterol (T-Ch) and higher HDL-cholesterol (HDL-Ch) in serum. These beneficial effects of OMW biophenols were confirmed by histological findings in hepatic, renal and pancreatic tissues of diabetic rats. This study demonstrates for the first time that OMW polyphenols and especially (HT) are efficient in inhibiting hyperglycemia and oxidative stress induced by diabetes and suggests that administration of HT may be helpful in the prevention of diabetic complications associated with oxidative stress.
本研究旨在评估从橄榄渣（OMW）中提取的酚类提取物和纯化的羟基酪醇（HT）对四氧嘧啶诱导的糖尿病大鼠氧化应激和高血糖的影响。使用乙酸乙酯提取OMW中的生物酚。通过固相萃取（SPE）实验对获得的提取物进行分馏，得到两个馏分：（F1）和（F2）。高效液相色谱-紫外（HPLC-UV）和高效液相色谱-质谱（HPLC-MS）分析表明，（F1）由已知的OMW单体酚类物质组成，主要是羟基酪醇（HT），而（F2）含有低聚和聚合酚类物质，如毛蕊花糖苷和橄榄苦苷。使用硅胶柱色谱和硅胶薄层层析技术从（F1）中纯化出（HT）。在孵育的胰腺中，补充OMW馏分可增强胰岛素分泌。给糖尿病大鼠施用OMW提取物馏分（F1）和（F2）以及纯化的（HT），可导致血浆葡萄糖水平降低，肝脏和肾脏中的肾超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPX）活性增加。此外，明显观察到对糖尿病大鼠肝脏和肾脏毒性的保护作用。此外，观察到肝脏和肾脏指标毒性显著降低，即碱性磷酸酶（ALP）、天冬氨酸和乳酸转氨酶（AST和ALT）活性以及硫代巴比妥酸反应性物质（TBARs）、总胆红素和直接胆红素、肌酐和尿素水平。此外，（F1）、（F2），尤其是（HT）可降低血清中的甘油三酯（TG）、总胆固醇（T-Ch）并提高高密度脂蛋白胆固醇（HDL-Ch）水平。糖尿病大鼠肝脏、肾脏和胰腺组织的组织学结果证实了OMW生物酚的这些有益作用。本研究首次证明，OMW多酚，尤其是（HT），在抑制糖尿病诱导的高血糖和氧化应激方面是有效的，并表明施用HT可能有助于预防与氧化应激相关的糖尿病并发症。
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