Hong Rui, Fischer Nicholas O, Verma Ayush, Goodman Catherine M, Emrick Todd, Rotello Vincent M
Department of Chemistry, University of Massachusetts, Amherst, Massachusetts 01003, USA.
J Am Chem Soc. 2004 Jan 28;126(3):739-43. doi: 10.1021/ja037470o.
Thioalkyl and thioalkylated oligo(ethylene glycol) (OEG) ligands with chain-end functionality were used to fabricate water-soluble CdSe nanoparticle scaffolds. Surface recognition of chymotrypsin (ChT) was achieved using these functionalized nanoparticle scaffolds, with three levels of interaction demonstrated: no interaction (OEG terminated with hydroxyl group), inhibition with denaturation (carboxylate-terminated thioalkyl ligands), and inhibition with retention of structure (carboxylate-terminated OEG). The latter process was reversible upon an increase in ionic strength, with essentially complete restoration of enzymatic activity.
具有链端功能的硫代烷基和硫代烷基化聚乙二醇（OEG）配体被用于制备水溶性CdSe纳米颗粒支架。利用这些功能化纳米颗粒支架实现了对胰凝乳蛋白酶（ChT）的表面识别，展示了三种相互作用水平：无相互作用（羟基封端的OEG）、变性抑制（羧基封端的硫代烷基配体）以及结构保留抑制（羧基封端的OEG）。后一过程在离子强度增加时是可逆的，酶活性基本完全恢复。
