Zhou Wen, Yamazaki Shiho, Yamakawa Akiyo, Ohtani Masahiro, Ito Yoshiyuki, Keida Yoshihide, Higashi Hideaki, Hatakeyama Masanori, Si Jianmin, Azuma Takeshi
Second Department of Internal Medicine, Fukui Medical University, Matsuoka-cho, Yoshida-gun, Fukui 910-1193, Japan.
FEMS Immunol Med Microbiol. 2004 Jan 15;40(1):81-7. doi: 10.1016/S0928-8244(03)00299-2.
It has been reported that Helicobacter pylori infection with the type I strain, which expresses the VacA and CagA antigens, is associated with duodenal ulcer. We examined the diversity of vacA and cagA genes in 143 isolates obtained from patients with duodenal ulcer or chronic gastritis in East Asia (two different areas of Japan, Fukui and Okinawa, and also in Hangzhou, China) by polymerase chain reaction (PCR) and sequence analysis. Diversities of cagA and vacA genes were detected in East Asia. The prevalence of cagA-positive H. pylori was significantly different between Fukui and Okinawa (P=0.0032). The prevalence of Western type CagA was significantly higher in Okinawa than in Fukui (P<0.0001). However, there was no significant association between the genotype of cagA and clinical outcome. In Japan, the predominant vacA genotype was s1c/m1b. In contrast, in Hangzhou, the predominant vacA genotype was s1c/m2, and they were all East Asian CagA-positive. These findings suggest that a distinct distribution of the vacA and cagA genotypes is present in East Asia, regardless of clinical outcome.
据报道，感染表达空泡毒素A（VacA）和细胞毒素相关基因A（CagA）抗原的I型菌株幽门螺杆菌与十二指肠溃疡有关。我们通过聚合酶链反应（PCR）和序列分析，检测了从东亚地区（日本福井和冲绳的两个不同地区以及中国杭州）十二指肠溃疡或慢性胃炎患者中分离出的143株菌株的vacA和cagA基因的多样性。在东亚地区检测到了cagA和vacA基因的多样性。福井和冲绳之间cagA阳性幽门螺杆菌的流行率存在显著差异（P = 0.0032）。冲绳地区西方型CagA的流行率显著高于福井（P < 0.0001）。然而，cagA基因型与临床结果之间没有显著关联。在日本，主要的vacA基因型是s1c/m1b。相比之下，在杭州，主要的vacA基因型是s1c/m2，并且它们都是东亚CagA阳性。这些发现表明，无论临床结果如何，东亚地区都存在vacA和cagA基因型的独特分布。
