Caner Vildan, Yilmaz Mustafa, Yonetci Nadir, Zencir Sevil, Karagenc Nedim, Kaleli Ilknur, Bagci Huseyin
Department of Medical Biology, School of Medicine, Pamukkale University, Kinikli, Denizli, Turkey.
World J Gastroenterol. 2007 May 14;13(18):2581-5. doi: 10.3748/wjg.v13.i18.2581.
To characterize and compare genotype profiles of H pylori strains isolated from patients with chronic gastritis and duodenal ulcer in western part of Turkey.
A total of 46 patients [30 chronic gastritis (CG) and 16 duodenal ulcer (DU)] who had undergone endoscopy because of dyspeptic complaints were studied. The antral biopsy specimens were evaluated for the presence of H pylori by rapid urease test and culture, and the genotype profiles were determined by real-time PCR.
The cagA gene was observed in 43 (93.5%) isolates. The vacA s1m2 genotype was the predominant subtype, found in 63.3% and 68.7% of isolates in patients with CG and DU, respectively. Twenty (66.6%) isolates from patients with CG were iceA2 positive while the iceA1 was predominant in those with DU (68.8%). In terms of the association of the iceA alleles to other genes, both alleles were significantly associated with the cagA vacA s1m2 genotype.
The prevalent circulating genotypes in CG and DU were cagA vacA s1m2 iceA2 and cagA vacA s1m2 iceA1 genotype, respectively. It was found that cagA vacA s1m2 genotype seems to be common virulence factors in both CG and DU while iceA alleles show specificity for gastroduodenal pathologies in this study.
对从土耳其西部慢性胃炎和十二指肠溃疡患者中分离出的幽门螺杆菌菌株的基因型谱进行特征分析和比较。
共研究了46例因消化不良症状接受内镜检查的患者[30例慢性胃炎（CG）和16例十二指肠溃疡（DU）]。通过快速尿素酶试验和培养评估胃窦活检标本中幽门螺杆菌的存在情况，并通过实时聚合酶链反应确定基因型谱。
在43株（93.5%）分离株中观察到cagA基因。vacA s1m2基因型是主要亚型，分别在CG患者和DU患者的63.3%和68.7%的分离株中发现。来自CG患者的20株（66.6%）分离株iceA2呈阳性，而iceA1在DU患者中占主导地位（68.8%）。就iceA等位基因与其他基因的关联而言，两种等位基因均与cagA vacA s1m2基因型显著相关。
CG和DU中普遍流行的基因型分别是cagA vacA s1m2 iceA2和cagA vacA s1m2 iceA1基因型。研究发现，cagA vacA s1m2基因型似乎是CG和DU中常见的毒力因子，而在本研究中iceA等位基因对胃十二指肠病变具有特异性。
