Nguyen Trang Hoa, Ho Trang Thi My, Nguyen-Hoang Thien-Phuc, Qumar Shamsul, Pham Thuc Tran Dang, Bui Quy Nhuan, Bulach Dieter, Nguyen Thuy-Vy, Rahman Motiur
Oxford University Clinical Research Unit, 764 Vo Van Kiet Street, Ward 1, District 5, Ho Chi Minh City, Vietnam.
Department of Genetics, Faculty of Biology and Biotechnology, Ho Chi Minh University of Science, Ho Chi Minh City, Vietnam.
Gut Pathog. 2021 Sep 30;13(1):57. doi: 10.1186/s13099-021-00452-2.
The burden of Helicobacter pylori-induced gastric cancer varies based on predominant H. pylori population in various geographical regions. Vietnam is a high H. pylori burden country with the highest age-standardized incidence rate of gastric cancer (16.3 cases/100,000 for both sexes) in Southeast Asia, despite this data on the H. pylori population is scanty. We examined the global context of the endemic H. pylori population in Vietnam and present a contextual and comparative genomics analysis of 83 H. pylori isolates from patients in Vietnam.
There are at least two major H. pylori populations are circulating in symptomatic Vietnamese patients. The majority of the isolates (~ 80%, 66/83) belong to the hspEastAsia and the remaining belong to hpEurope population (~ 20%, 17/83). In total, 66 isolates (66/83) were cagA positive, 64 were hspEastAsia isolates and two were hpEurope isolates. Examination of the second repeat region revealed that most of the cagA genes were ABD type (63/66; 61 were hspEastAsia isolates and two were hpEurope isolates). The remaining three isolates (all from hspEastAsia isolates) were ABC or ABCC types. We also detected that 4.5% (3/66) cagA gene from hspEastAsia isolates contained EPIYA-like sequences, ESIYA at EPIYA-B segments. Analysis of the vacA allelic type revealed 98.8% (82/83) and 41% (34/83) of the strains harboured the s1 and m1 allelic variant, respectively; 34/83 carried both s1m1 alleles. The most frequent genotypes among the cagA positive isolates were vacA s1m1/cagA + and vacA s1m2/cagA + , accounting for 51.5% (34/66) and 48.5% (32/66) of the isolates, respectively.
There are two predominant lineages of H. pylori circulating in Vietnam; most of the isolates belong to the hspEastAsia population. The hpEurope population is further divided into two smaller clusters.
幽门螺杆菌引发的胃癌负担因不同地理区域幽门螺杆菌的主要种群而异。越南是幽门螺杆菌负担较重的国家，在东南亚地区胃癌的年龄标准化发病率最高（男女均为16.3例/10万），尽管关于幽门螺杆菌种群的数据很少。我们研究了越南地方性幽门螺杆菌种群的全球背景，并对来自越南患者的83株幽门螺杆菌分离株进行了背景和比较基因组学分析。
在有症状的越南患者中，至少有两个主要的幽门螺杆菌种群在传播。大多数分离株（约80%，66/83）属于hspEastAsia种群，其余的属于hpEurope种群（约20%，17/83）。总共有66株分离株（66/83）cagA呈阳性，其中64株是hspEastAsia分离株，2株是hpEurope分离株。对第二个重复区域的检查显示，大多数cagA基因是ABD型（63/66；61株是hspEastAsia分离株，2株是hpEurope分离株）。其余三株分离株（均来自hspEastAsia分离株）是ABC或ABCC型。我们还检测到，hspEastAsia分离株中有4.5%（3/66）的cagA基因在EPIYA - B区段含有类EPIYA序列ESiya。对vacA等位基因类型的分析显示，分别有98.8%（82/83）和41%（34/83）的菌株携带s1和m1等位基因变体；34/83同时携带s1m1等位基因。cagA阳性分离株中最常见的基因型是vacA s1m1/cagA +和vacA s1m2/cagA +，分别占分离株的51.5%（34/66）和48.5%（32/66）。
在越南有两个主要的幽门螺杆菌谱系在传播；大多数分离株属于hspEastAsia种群。hpEurope种群进一步分为两个较小的簇。
