哺乳动物神经祖细胞基因表达程序的分析
An analysis of the gene expression program of mammalian neural progenitor cells.
作者信息
Livesey F J, Young T L, Cepko C L
机构信息
Department of Genetics and Howard Hughes Medical Institute, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA.
出版信息
Proc Natl Acad Sci U S A. 2004 Feb 3;101(5):1374-9. doi: 10.1073/pnas.0307014101. Epub 2004 Jan 20.
Abstract
A diverse range of neural cell types is generated from a pool of dividing stem and progenitor cells in an orderly manner during development. Little is known of the molecular and cellular biology underpinning the intrinsic control of this process. We have used a nonbiased method to purify populations of neural progenitor cells from the murine CNS to characterize the gene expression program of mammalian retinal progenitor cells. Analysis of these data led to the identification of a core set of >800 transcripts enriched in retinal progenitor cells compared to both their immediate postmitotic progeny and to differentiated neurons. This core set was found to be shared by progenitors in other regions of the developing CNS, with important regional differences in key functional families. In addition to providing an expression fingerprint of this cell type, this set highlights several key aspects of progenitor biology.
摘要
在发育过程中，多种神经细胞类型由一群不断分裂的干细胞和祖细胞有序产生。对于这一过程内在控制的分子和细胞生物学，我们了解甚少。我们采用了一种无偏向性的方法，从鼠类中枢神经系统中纯化神经祖细胞群体，以表征哺乳动物视网膜祖细胞的基因表达程序。对这些数据的分析导致鉴定出一组核心的800多个转录本，与它们刚完成有丝分裂的子代细胞以及分化的神经元相比，这些转录本在视网膜祖细胞中富集。发现这一核心集在发育中的中枢神经系统其他区域的祖细胞中也有共享，但在关键功能家族中存在重要的区域差异。除了提供这种细胞类型的表达指纹外，这一数据集还突出了祖细胞生物学的几个关键方面。
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