终止密码子通读中主要的5'决定因素涉及两个相邻的腺嘌呤。
The major 5' determinant in stop codon read-through involves two adjacent adenines.
作者信息
Tork Sanaa, Hatin Isabelle, Rousset Jean-Pierre, Fabret Céline
机构信息
CNRS UMR 8621, Institut de Génétique et Microbiologie, Université Paris-Sud, 91405 Orsay Cedex, France.
出版信息
Nucleic Acids Res. 2004 Jan 21;32(2):415-21. doi: 10.1093/nar/gkh201. Print 2004.
Abstract
The aim of this approach was to identify the major determinants, located at the 5' end of the stop codon, that modulate translational read-through in Saccharomyces cerevisiae. We developed a library of oligonucleotides degenerate at the six positions immediately upstream of the termination codon, cloned in the ADE2 reporter gene. Variations at these positions modulated translational read-through efficiency approximately 16-fold. The major effect was imposed by the two nucleotides immediately upstream of the stop codon. We showed that this effect was neither mediated by the last amino acid residues present in the polypeptide chain nor by the tRNA present in the ribosomal P site. We propose that the mRNA structure, depending on the nucleotides in the P site, is the main 5' determinant of read-through efficiency.
摘要
这种方法的目的是确定位于终止密码子5'端的主要决定因素，这些因素可调节酿酒酵母中的翻译通读。我们构建了一个寡核苷酸文库，该文库在终止密码子上游紧邻的六个位置处具有简并性，并克隆到ADE2报告基因中。这些位置的变异可将翻译通读效率调节约16倍。主要影响是由终止密码子上游紧邻的两个核苷酸施加的。我们表明，这种影响既不是由多肽链中存在的最后氨基酸残基介导的，也不是由核糖体P位点中存在的tRNA介导的。我们提出，取决于P位点中核苷酸的mRNA结构是通读效率的主要5'决定因素。
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