Dom34:Hbs1 促进亚基解离和肽酰-tRNA 脱落，以启动无意义衰变。
Dom34:Hbs1 promotes subunit dissociation and peptidyl-tRNA drop-off to initiate no-go decay.
机构信息
Howard Hughes Medical Institute and Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
出版信息
Science. 2010 Oct 15;330(6002):369-72. doi: 10.1126/science.1192430.
Abstract
No-go decay (NGD) is one of several messenger RNA (mRNA) surveillance systems dedicated to the removal of defective mRNAs from the available pool. Two interacting factors, Dom34 and Hbs1, are genetically implicated in NGD in yeast. Using a reconstituted yeast translation system, we show that Dom34:Hbs1 interacts with the ribosome to promote subunit dissociation and peptidyl-tRNA drop-off. Our data further indicate that these recycling activities are shared by the homologous translation termination factor complex eRF1:eRF3, suggesting a common ancestral function. Because Dom34:Hbs1 activity exhibits no dependence on either peptide length or A-site codon identity, we propose that this quality-control system functions broadly to recycle ribosomes throughout the translation cycle whenever stalls occur.
摘要
无终止衰变（NGD）是几种专门用于从可用池中去除有缺陷的 mRNA 的信使 RNA（mRNA）监测系统之一。两个相互作用的因子，Dom34 和 Hbs1，在酵母中与 NGD 在遗传上有关。使用重建的酵母翻译系统，我们表明 Dom34：Hbs1 与核糖体相互作用，以促进亚基解离和肽酰-tRNA 脱落。我们的数据进一步表明，这些回收活动由同源的翻译终止因子复合物 eRF1:eRF3 共享，表明存在共同的祖先功能。由于 Dom34：Hbs1 的活性不依赖于肽的长度或 A 位密码子的身份，我们提出这个质量控制系统在翻译周期中只要发生停顿就会广泛地循环利用核糖体。
相似文献
1
Dom34:Hbs1 promotes subunit dissociation and peptidyl-tRNA drop-off to initiate no-go decay.Dom34:Hbs1 促进亚基解离和肽酰-tRNA 脱落，以启动无意义衰变。
Science. 2010 Oct 15;330(6002):369-72. doi: 10.1126/science.1192430.
2
Dom34:hbs1 plays a general role in quality-control systems by dissociation of a stalled ribosome at the 3' end of aberrant mRNA.Dom34:hbs1 通过在异常 mRNA 的 3' 末端解离stalled 核糖体，在质量控制系统中发挥一般作用。
Mol Cell. 2012 May 25;46(4):518-29. doi: 10.1016/j.molcel.2012.03.013. Epub 2012 Apr 11.
3
Protein quality control systems associated with no-go and nonstop mRNA surveillance in yeast.与酵母中无终止和非终止 mRNA 监测相关的蛋白质质量控制系统。
Genes Cells. 2014 Jan;19(1):1-12. doi: 10.1111/gtc.12106. Epub 2013 Nov 21.
4
Structure of yeast Dom34: a protein related to translation termination factor Erf1 and involved in No-Go decay.酵母Dom34的结构：一种与翻译终止因子Erf1相关且参与无义介导的mRNA降解的蛋白质。
J Biol Chem. 2008 Mar 14;283(11):7145-54. doi: 10.1074/jbc.M708224200. Epub 2008 Jan 7.
5
Structural insights into ribosomal rescue by Dom34 and Hbs1 at near-atomic resolution.在近原子分辨率下解析 Dom34 和 Hbs1 介导的核糖体救援结构。
Nat Commun. 2016 Dec 20;7:13521. doi: 10.1038/ncomms13521.
6
Dom34-Hbs1 mediated dissociation of inactive 80S ribosomes promotes restart of translation after stress.Dom34-Hbs1 介导的无活性 80S 核糖体解离促进应激后翻译的重新启动。
EMBO J. 2014 Feb 3;33(3):265-76. doi: 10.1002/embj.201386123. Epub 2014 Jan 14.
7
Structure of the no-go mRNA decay complex Dom34-Hbs1 bound to a stalled 80S ribosome.无终止 mRNA 降解复合物 Dom34-Hbs1 与停滞的 80S 核糖体结合的结构。
Nat Struct Mol Biol. 2011 Jun;18(6):715-20. doi: 10.1038/nsmb.2057. Epub 2011 May 29.
8
Dissection of Dom34-Hbs1 reveals independent functions in two RNA quality control pathways.Dom34-Hbs1 的结构解析揭示了其在两条 RNA 质量控制途径中具有独立的功能。
Nat Struct Mol Biol. 2010 Dec;17(12):1446-52. doi: 10.1038/nsmb.1963. Epub 2010 Nov 21.
9
Roles of dom34:hbs1 in nonstop protein clearance from translocators for normal organelle protein influx.Dom34:hbs1 在非停蛋白清除中的作用：用于正常细胞器蛋白流入的转运蛋白。
Cell Rep. 2012 Sep 27;2(3):447-53. doi: 10.1016/j.celrep.2012.08.010. Epub 2012 Sep 13.
10
Structure of the Dom34-Hbs1 complex and implications for no-go decay.Dom34-Hbs1 复合物的结构及其对无终止衰变的影响。
Nat Struct Mol Biol. 2010 Oct;17(10):1233-40. doi: 10.1038/nsmb.1922. Epub 2010 Oct 3.
引用本文的文献
1
Comparative CRISPRi screens reveal a human stem cell dependence on mRNA translation-coupled quality control.比较性CRISPR干扰筛选揭示了人类干细胞对mRNA翻译偶联质量控制的依赖性。
Nat Struct Mol Biol. 2025 Jul 11. doi: 10.1038/s41594-025-01616-3.
2
tRNA synthetase activity is required for stress granule and P-body assembly.应激颗粒和P小体组装需要tRNA合成酶活性。
bioRxiv. 2025 Mar 13:2025.03.10.642431. doi: 10.1101/2025.03.10.642431.
3
RNA damage and its implications in genome stability.RNA损伤及其对基因组稳定性的影响。
DNA Repair (Amst). 2025 Mar;147:103821. doi: 10.1016/j.dnarep.2025.103821. Epub 2025 Mar 1.
4
RNA binding proteins (RBPs) on genetic stability and diseases.RNA结合蛋白（RBPs）与遗传稳定性及疾病
Glob Med Genet. 2024 Nov 30;12(1):100032. doi: 10.1016/j.gmg.2024.100032. eCollection 2025 Mar.
5
The ribosome as a platform to coordinate mRNA decay.核糖体作为协调信使核糖核酸衰变的平台。
Nucleic Acids Res. 2025 Feb 8;53(4). doi: 10.1093/nar/gkaf049.
6
Synthetic lethality of mRNA quality control complexes in cancer.癌症中mRNA质量控制复合体的合成致死性。
Nature. 2025 Feb;638(8052):1095-1103. doi: 10.1038/s41586-024-08398-6. Epub 2025 Feb 5.
7
Insect ribosome-rescuer Pelo-Hbs1 complex on sperm surface mediates paternal arbovirus transmission.精子表面的昆虫核糖体修复蛋白 Pelo-Hbs1 复合物介导亲代虫媒病毒传播。
Nat Commun. 2024 Aug 9;15(1):6817. doi: 10.1038/s41467-024-51020-6.
8
The kinase Rio1 and a ribosome collision-dependent decay pathway survey the integrity of 18S rRNA cleavage.激酶 Rio1 和核糖体碰撞依赖性衰变途径检测 18S rRNA 切割的完整性。
PLoS Biol. 2024 Apr 25;22(4):e3001767. doi: 10.1371/journal.pbio.3001767. eCollection 2024 Apr.
9
HBS1L deficiency causes retinal dystrophy in a child and in a mouse model associated with defective development of photoreceptor cells.HBS1L 缺乏症可导致儿童和小鼠模型的视网膜变性，与感光细胞发育缺陷有关。
Dis Model Mech. 2024 Aug 1;17(8). doi: 10.1242/dmm.050557. Epub 2024 Jul 30.
10
Dysregulated ribosome quality control in human diseases.人类疾病中核糖体质量控制失调
FEBS J. 2025 Mar;292(5):936-959. doi: 10.1111/febs.17217. Epub 2024 Jul 1.
本文引用的文献
1
Ribosome recycling step in yeast cytoplasmic protein synthesis is catalyzed by eEF3 and ATP.核糖体循环步骤在酵母细胞质蛋白合成中由 eEF3 和 ATP 催化。
Proc Natl Acad Sci U S A. 2010 Jun 15;107(24):10854-9. doi: 10.1073/pnas.1006247107. Epub 2010 Jun 1.
2
Mechanism of eIF6-mediated inhibition of ribosomal subunit joining.eIF6 介导的核糖体亚基连接抑制机制。
J Biol Chem. 2010 May 14;285(20):14848-14851. doi: 10.1074/jbc.C109.096057. Epub 2010 Mar 31.
3
The role of ABCE1 in eukaryotic posttermination ribosomal recycling.ABCE1 在真核生物终止后核糖体循环中的作用。
Mol Cell. 2010 Jan 29;37(2):196-210. doi: 10.1016/j.molcel.2009.12.034.
4
The iron-sulphur protein RNase L inhibitor functions in translation termination.铁硫蛋白 RNase L 抑制剂在翻译终止中发挥作用。
EMBO Rep. 2010 Mar;11(3):214-9. doi: 10.1038/embor.2009.272. Epub 2010 Jan 8.
5
Immature small ribosomal subunits can engage in translation initiation in Saccharomyces cerevisiae.不成熟的小核糖体亚基可在酿酒酵母中参与翻译起始。
EMBO J. 2010 Jan 6;29(1):80-92. doi: 10.1038/emboj.2009.307. Epub 2009 Nov 5.
6
A convergence of rRNA and mRNA quality control pathways revealed by mechanistic analysis of nonfunctional rRNA decay.通过对无功能rRNA衰变的机制分析揭示的rRNA和mRNA质量控制途径的趋同。
Mol Cell. 2009 May 14;34(4):440-50. doi: 10.1016/j.molcel.2009.04.017.
7
Hypusine-containing protein eIF5A promotes translation elongation.含hypusine的蛋白质eIF5A促进翻译延伸。
Nature. 2009 May 7;459(7243):118-21. doi: 10.1038/nature08034.
8
Analysis of Dom34 and its function in no-go decay.Dom34分析及其在无义衰变中的功能。
Mol Biol Cell. 2009 Jul;20(13):3025-32. doi: 10.1091/mbc.e09-01-0028. Epub 2009 May 6.
9
Kinetic and thermodynamic analysis of the role of start codon/anticodon base pairing during eukaryotic translation initiation.真核生物翻译起始过程中起始密码子/反密码子碱基配对作用的动力学和热力学分析。
RNA. 2009 Jan;15(1):138-52. doi: 10.1261/rna.1318509. Epub 2008 Nov 24.
10
Evolution of nonstop, no-go and nonsense-mediated mRNA decay and their termination factor-derived components.无义、无-go和无义介导的mRNA降解的演变及其终止因子衍生成分。
BMC Evol Biol. 2008 Oct 23;8:290. doi: 10.1186/1471-2148-8-290.
Zotero 插件