Howard Hughes Medical Institute and Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Science. 2010 Oct 15;330(6002):369-72. doi: 10.1126/science.1192430.
No-go decay (NGD) is one of several messenger RNA (mRNA) surveillance systems dedicated to the removal of defective mRNAs from the available pool. Two interacting factors, Dom34 and Hbs1, are genetically implicated in NGD in yeast. Using a reconstituted yeast translation system, we show that Dom34:Hbs1 interacts with the ribosome to promote subunit dissociation and peptidyl-tRNA drop-off. Our data further indicate that these recycling activities are shared by the homologous translation termination factor complex eRF1:eRF3, suggesting a common ancestral function. Because Dom34:Hbs1 activity exhibits no dependence on either peptide length or A-site codon identity, we propose that this quality-control system functions broadly to recycle ribosomes throughout the translation cycle whenever stalls occur.
无终止衰变（NGD）是几种专门用于从可用池中去除有缺陷的 mRNA 的信使 RNA（mRNA）监测系统之一。两个相互作用的因子，Dom34 和 Hbs1，在酵母中与 NGD 在遗传上有关。使用重建的酵母翻译系统，我们表明 Dom34：Hbs1 与核糖体相互作用，以促进亚基解离和肽酰-tRNA 脱落。我们的数据进一步表明，这些回收活动由同源的翻译终止因子复合物 eRF1:eRF3 共享，表明存在共同的祖先功能。由于 Dom34：Hbs1 的活性不依赖于肽的长度或 A 位密码子的身份，我们提出这个质量控制系统在翻译周期中只要发生停顿就会广泛地循环利用核糖体。
