• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

使用原子力显微镜（AFM）对活细胞进行力和顺应性测量。

Force and Compliance Measurements on Living Cells Using Atomic Force Microscopy (AFM).

作者信息

Wojcikiewicz Ewa P., Zhang Xiaohui, Moy Vincent T.

机构信息

Department of Physiology and Biophysics, University of Miami School of Medicine. 1600 NW 10th Avenue, Miami, FL 33136. USA.

出版信息

Biol Proced Online. 2004;6:1-9. doi: 10.1251/bpo67. Epub 2004 Jan 15.

DOI:10.1251/bpo67
PMID:14737221
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC315478/
Abstract

We describe the use of atomic force microscopy (AFM) in studies of cell adhesion and cell compliance. Our studies use the interaction between leukocyte function associated antigen-1 (LFA-1)/intercellular adhesion molecule-1 (ICAM-1) as a model system. The forces required to unbind a single LFA-1/ICAM-1 bond were measured at different loading rates. This data was used to determine the dynamic strength of the LFA-1/ICAM-1 complex and characterize the activation potential that this complex overcomes during its breakage. Force measurements acquired at the multiple- bond level provided insight about the mechanism of cell adhesion. In addition, the AFM was used as a microindenter to determine the mechanical properties of cells. The applications of these methods are described using data from a previous study.

摘要

我们描述了原子力显微镜（AFM）在细胞黏附和细胞顺应性研究中的应用。我们的研究使用白细胞功能相关抗原-1（LFA-1）/细胞间黏附分子-1（ICAM-1）之间的相互作用作为模型系统。在不同加载速率下测量了分离单个LFA-1/ICAM-1键所需的力。该数据用于确定LFA-1/ICAM-1复合物的动态强度，并表征该复合物在断裂过程中克服的激活电位。在多键水平上进行的力测量提供了有关细胞黏附机制的见解。此外，AFM被用作微压痕仪来确定细胞的力学性能。使用先前研究的数据描述了这些方法的应用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f402/315478/933e2c8f886e/bpo_v6_p1_m67f5lg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f402/315478/42cfbe3078c7/bpo_v6_p1_m67f1lg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f402/315478/0f8b68ff8d16/bpo_v6_p1_m67f2lg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f402/315478/593462a88f80/bpo_v6_p1_m67f3lg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f402/315478/21b2f2349d9f/bpo_v6_p1_m67f4lg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f402/315478/933e2c8f886e/bpo_v6_p1_m67f5lg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f402/315478/42cfbe3078c7/bpo_v6_p1_m67f1lg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f402/315478/0f8b68ff8d16/bpo_v6_p1_m67f2lg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f402/315478/593462a88f80/bpo_v6_p1_m67f3lg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f402/315478/21b2f2349d9f/bpo_v6_p1_m67f4lg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f402/315478/933e2c8f886e/bpo_v6_p1_m67f5lg.jpg

相似文献

1
Force and Compliance Measurements on Living Cells Using Atomic Force Microscopy (AFM).使用原子力显微镜（AFM）对活细胞进行力和顺应性测量。
Biol Proced Online. 2004;6:1-9. doi: 10.1251/bpo67. Epub 2004 Jan 15.
2
Force spectroscopy of the leukocyte function-associated antigen-1/intercellular adhesion molecule-1 interaction.白细胞功能相关抗原-1/细胞间黏附分子-1相互作用的力谱分析
Biophys J. 2002 Oct;83(4):2270-9. doi: 10.1016/S0006-3495(02)73987-8.
3
Force spectroscopy of LFA-1 and its ligands, ICAM-1 and ICAM-2.淋巴细胞功能相关抗原-1（LFA-1）及其配体细胞间黏附分子-1（ICAM-1）和细胞间黏附分子-2（ICAM-2）的力谱分析
Biomacromolecules. 2006 Nov;7(11):3188-95. doi: 10.1021/bm060559c.
4
Contributions of molecular binding events and cellular compliance to the modulation of leukocyte adhesion.分子结合事件和细胞顺应性对白细胞黏附调节的作用。
J Cell Sci. 2003 Jun 15;116(Pt 12):2531-9. doi: 10.1242/jcs.00465. Epub 2003 May 6.
5
Ligand-specific binding forces of LFA-1 and Mac-1 in neutrophil adhesion and crawling.中性粒细胞黏附和爬行中 LFA-1 和 Mac-1 的配体特异性结合力。
Mol Biol Cell. 2018 Feb 15;29(4):408-418. doi: 10.1091/mbc.E16-12-0827. Epub 2017 Dec 27.
6
Atomic force microscopy measurements of protein-ligand interactions on living cells.基于原子力显微镜的活细胞上蛋白质-配体相互作用的测量
Methods Mol Biol. 2005;305:439-50. doi: 10.1385/1-59259-912-5:439.
7
Substrate stiffness of endothelial cells directs LFA-1/ICAM-1 interaction: A physical trigger of immune-related diseases?内皮细胞的底物硬度指导淋巴细胞功能相关抗原-1/细胞间黏附分子-1相互作用：免疫相关疾病的物理触发因素？
Clin Hemorheol Microcirc. 2016;61(4):633-43. doi: 10.3233/CH-151951.
8
Role of ICAM-1 polymorphisms (G241R, K469E) in mediating its single-molecule binding ability: atomic force microscopy measurements on living cells.细胞间黏附分子-1 多态性（G241R、K469E）在调节其单分子结合能力中的作用：活细胞的原子力显微镜测量。
Biochem Biophys Res Commun. 2014 Jun 13;448(4):372-8. doi: 10.1016/j.bbrc.2014.04.113. Epub 2014 Apr 30.
9
Interaction between single molecules of Mac-1 and ICAM-1 in living cells: an atomic force microscopy study.活细胞中Mac-1与细胞间黏附分子-1（ICAM-1）单分子之间的相互作用：一项原子力显微镜研究
Exp Cell Res. 2007 Oct 1;313(16):3497-504. doi: 10.1016/j.yexcr.2007.08.001. Epub 2007 Aug 9.
10
Costimulation of T cell receptor/CD3-mediated activation of resting human CD4+ T cells by leukocyte function-associated antigen-1 ligand intercellular cell adhesion molecule-1 involves prolonged inositol phospholipid hydrolysis and sustained increase of intracellular Ca2+ levels.白细胞功能相关抗原-1配体细胞间黏附分子-1对静止人类CD4⁺T细胞的T细胞受体/CD3介导的激活的共刺激涉及延长的肌醇磷脂水解和细胞内Ca²⁺水平的持续升高。
J Immunol. 1992 Dec 15;149(12):3872-80.

引用本文的文献

1
Cooperative control of a DNA origami force sensor.DNA折纸力传感器的协同控制
Sci Rep. 2024 Feb 19;14(1):4132. doi: 10.1038/s41598-024-53841-3.
2
Single-Cell Analysis with Silver-Coated Pipette by Combined SERS and SICM.银涂层移液器的单细胞分析：结合 SERS 和 SICM 技术。
Cells. 2023 Oct 25;12(21):2521. doi: 10.3390/cells12212521.
3
Biophysical investigation of interactions between SARS-CoV-2 spike protein and neuropilin-1.SARS-CoV-2 刺突蛋白与神经纤毛蛋白-1 相互作用的生物物理研究。

本文引用的文献

1
Contributions of molecular binding events and cellular compliance to the modulation of leukocyte adhesion.分子结合事件和细胞顺应性对白细胞黏附调节的作用。
J Cell Sci. 2003 Jun 15;116(Pt 12):2531-9. doi: 10.1242/jcs.00465. Epub 2003 May 6.
2
Force spectroscopy of the leukocyte function-associated antigen-1/intercellular adhesion molecule-1 interaction.白细胞功能相关抗原-1/细胞间黏附分子-1相互作用的力谱分析
Biophys J. 2002 Oct;83(4):2270-9. doi: 10.1016/S0006-3495(02)73987-8.
3
Cell adhesion measured by force spectroscopy on living cells.
Protein Sci. 2023 Oct;32(10):e4773. doi: 10.1002/pro.4773.
4
A mathematical model of fibrinogen-mediated erythrocyte-erythrocyte adhesion.纤维蛋白原介导的红细胞-红细胞黏附的数学模型。
Commun Biol. 2023 Feb 17;6(1):192. doi: 10.1038/s42003-023-04560-4.
5
Sensing Cells-Peptide Hydrogel Interaction In Situ via Scanning Ion Conductance Microscopy.通过扫描离子电导显微镜原位感知细胞-肽水凝胶相互作用。
Cells. 2022 Dec 19;11(24):4137. doi: 10.3390/cells11244137.
6
Atomic Force Microscopy (AFM) on Biopolymers and Hydrogels for Biotechnological Applications-Possibilities and Limits.用于生物技术应用的生物聚合物和水凝胶的原子力显微镜——可能性与局限性
Polymers (Basel). 2022 Mar 21;14(6):1267. doi: 10.3390/polym14061267.
7
Dynamic alteration of poroelastic attributes as determinant membrane nanorheology for endocytosis of organ specific targeted gold nanoparticles.动态改变多孔弹性特性作为决定细胞膜纳米流变特性的因素，用于细胞器靶向金纳米粒子的内吞作用。
J Nanobiotechnology. 2022 Feb 8;20(1):74. doi: 10.1186/s12951-022-01276-1.
8
A comparative study of the adhesion of biosynthesized gold and conjugated gold/prodigiosin nanoparticles to triple negative breast cancer cells.生物合成金和共轭金/血晶素纳米颗粒对三阴性乳腺癌细胞黏附的比较研究。
J Mater Sci Mater Med. 2017 Aug 17;28(9):143. doi: 10.1007/s10856-017-5943-2.
9
Quantifying Molecular-Level Cell Adhesion on Electroactive Conducting Polymers using Electrochemical-Single Cell Force Spectroscopy.使用电化学单细胞力谱法对电活性导电聚合物上的分子水平细胞粘附进行定量分析。
Sci Rep. 2015 Sep 3;5:13334. doi: 10.1038/srep13334.
10
Increasing throughput of AFM-based single cell adhesion measurements through multisubstrate surfaces.通过多底物表面提高基于原子力显微镜的单细胞黏附测量的通量。
Beilstein J Nanotechnol. 2015 Jan 14;6:157-66. doi: 10.3762/bjnano.6.15. eCollection 2015.
通过对活细胞进行力谱分析来测量细胞黏附。
Methods Cell Biol. 2002;68:91-114. doi: 10.1016/s0091-679x(02)68006-9.
4
Probing the relation between force--lifetime--and chemistry in single molecular bonds.探究单分子键中力、寿命与化学性质之间的关系。
Annu Rev Biophys Biomol Struct. 2001;30:105-28. doi: 10.1146/annurev.biophys.30.1.105.
5
Biomolecular interactions measured by atomic force microscopy.通过原子力显微镜测量的生物分子相互作用。
Biophys J. 2000 Dec;79(6):3267-81. doi: 10.1016/S0006-3495(00)76559-3.
6
Avidity regulation of integrins: the driving force in leukocyte adhesion.整合素的亲和力调节：白细胞黏附中的驱动力。
Curr Opin Cell Biol. 2000 Oct;12(5):542-7. doi: 10.1016/s0955-0674(00)00129-0.
7
Energy landscape of streptavidin-biotin complexes measured by atomic force microscopy.通过原子力显微镜测量的链霉亲和素-生物素复合物的能量景观。
Biochemistry. 2000 Aug 22;39(33):10219-23. doi: 10.1021/bi992715o.
8
Discrete interactions in cell adhesion measured by single-molecule force spectroscopy.通过单分子力谱法测量细胞黏附中的离散相互作用。
Nat Cell Biol. 2000 Jun;2(6):313-7. doi: 10.1038/35014000.
9
NMR and mutagenesis evidence for an I domain allosteric site that regulates lymphocyte function-associated antigen 1 ligand binding.核磁共振和诱变证据表明存在一个调节淋巴细胞功能相关抗原1配体结合的I结构域变构位点。
Proc Natl Acad Sci U S A. 2000 May 9;97(10):5231-6. doi: 10.1073/pnas.97.10.5231.
10
Macrophage-enriched myristoylated alanine-rich C kinase substrate and its phosphorylation is required for the phorbol ester-stimulated diffusion of beta 2 integrin molecules.
J Biol Chem. 2000 Jun 30;275(26):20217-22. doi: 10.1074/jbc.M909129199.