Xili L, Chengjiany B, Eryi X, Reiming S, Yuengming W, Haodong S, Zhiyian H
Department of Public Health, Tianjin Medical College, People's Republic of China.
Food Chem Toxicol. 1992 Nov;30(11):957-65. doi: 10.1016/0278-6915(92)90181-j.
Groups of 45 male and 45 female inbred Wistar rats were given diets containing stevioside (85% pure) at 0, 0.2, 0.6 or 1.2% for 2 yr. After 6, 12 and 24 months, five rats from each group were killed for haematological and clinical biochemical tests. Growth, food utilization and consumption, general appearance and mortality were similar in treated and control groups. The mean lifespan of rats given stevioside was not significantly different from that of the controls. No treatment-related changes were observed in haematological, urinary or clinical biochemical values at any stage of the study. The incidence and severity of non-neoplastic and neoplastic changes were unrelated to the level of stevioside in the diet. The maximum no-observed-effect level of stevioside was 1.2%, and an acceptable daily intake of stevioside for humans of 7.938 mg/kg body weight/day is suggested.
将45只雄性和45只雌性近交系Wistar大鼠分为几组，分别给予含0%、0.2%、0.6%或1.2%甜菊糖苷（纯度85%）的饲料，持续2年。在6个月、12个月和24个月后，每组处死5只大鼠进行血液学和临床生化检测。处理组和对照组在生长、食物利用率和消耗量、总体外观及死亡率方面相似。给予甜菊糖苷的大鼠平均寿命与对照组无显著差异。在研究的任何阶段，血液学、尿液或临床生化值均未观察到与处理相关的变化。非肿瘤性和肿瘤性变化的发生率及严重程度与饲料中甜菊糖苷的水平无关。甜菊糖苷的最大无观察到效应水平为1.2%，并建议人类甜菊糖苷的可接受每日摄入量为7.938毫克/千克体重/天。
