Dumas Jean-François, Roussel Damien, Simard Gilles, Douay Olivier, Foussard Françoise, Malthiery Yves, Ritz Patrick
INSERM EMI-U 00.18, Laboratoire de Biochimie et de Biologie Moléculaire, Medecine B, CHU, F-49033 Angers Cedex 01, France.
Biochim Biophys Acta. 2004 Jan 22;1670(2):126-31. doi: 10.1016/j.bbagen.2003.11.002.
To examine the effect of 50% food restriction over a period of 3 days on mitochondrial energy metabolism, liver mitochondria were isolated from ad libitum and food-restricted rats. Mitochondrial enzyme activities and oxygen consumption were assessed spectrophotometrically and polarographically. With regard to body weight loss (-5%), food restriction decreased the liver to body mass ratio by 7%. Moreover, in food-restricted rats, liver mitochondria displayed diminished state 3 (-30%), state 4-oligomycin (-26%) and uncoupled state (-24%) respiration rates in the presence of succinate. Furthermore, "top-down" elasticity showed that these decreases were due to an inactivation of reactions involved in substrate oxidation. Therefore, it appears that rats not only adapt to food restriction through simple passive mechanisms, such as liver mass loss, but also through decreased mitochondrial energetic metabolism.
为研究3天50%食物限制对线粒体能量代谢的影响，从自由摄食和食物限制的大鼠中分离出肝脏线粒体。采用分光光度法和极谱法评估线粒体酶活性和氧消耗。关于体重减轻（-5%），食物限制使肝脏与体重之比降低了7%。此外，在食物限制的大鼠中，肝脏线粒体在琥珀酸存在下的状态3（-30%）、状态4-寡霉素（-26%）和非偶联状态（-24%）呼吸速率降低。此外，“自上而下”弹性分析表明，这些降低是由于底物氧化相关反应的失活。因此，似乎大鼠不仅通过简单的被动机制（如肝脏质量减轻）适应食物限制，还通过降低线粒体能量代谢来适应。
