DNA低甲基化在系统性红斑狼疮诱导中的可能作用：与人类内源性逆转录病毒转录的关系
Possible role of DNA hypomethylation in the induction of SLE: relationship to the transcription of human endogenous retroviruses.
作者信息
Ogasawara H, Okada M, Kaneko H, Hishikawa T, Sekigawa I, Hashimoto H
机构信息
Department of Internal Medicine and Rheumatology, Juntendo University School of Medicine, Tokyo, Japan.
出版信息
Clin Exp Rheumatol. 2003 Nov-Dec;21(6):733-8.
OBJECTIVE
We investigated the contribution of DNA methyltransferase activity to the transcription of human endogenous retroviruses (HERV), which have been reported to be a plausible causative agent for systemic lupus erythematosus (SLE).
METHODS
The reverse transcriptase-polymerase chain reaction (RT-PCR) and real-time quantitative-PCR (RQ-PCR) were used.
RESULTS
Our results indicated that treatment with 5-aza-deoxycytidine (5-aza C), a demethylating agent, increased the transcription of messenger RNA (mRNA) for HERV clone 4-1 and decreased mRNA for DNA methyltransferase-1 (DNMT-1; methylation-regulating enzyme) in peripheral blood mononuclear cells (PBMC) from normal individuals. Also, transcription of DNMT-1 mRNA in PBMC from patients with SLE was lower than in cells from normal controls.
CONCLUSION
DNA hypomethylation seems to play a significant role in the transcription of HERV clone 4-1 and may be related to the pathogenesis of SLE.
目的
我们研究了DNA甲基转移酶活性对人类内源性逆转录病毒（HERV）转录的作用，据报道，HERV可能是系统性红斑狼疮（SLE）的致病因素。
方法
采用逆转录聚合酶链反应（RT-PCR）和实时定量PCR（RQ-PCR）。
结果
我们的结果表明，用去甲基化剂5-氮杂脱氧胞苷（5-aza C）处理可增加正常个体外周血单个核细胞（PBMC）中HERV克隆4-1信使RNA（mRNA）的转录，并降低DNA甲基转移酶-1（DNMT-1；甲基化调节酶）的mRNA水平。此外，SLE患者PBMC中DNMT-1 mRNA的转录低于正常对照细胞。
结论
DNA低甲基化似乎在HERV克隆4-1的转录中起重要作用，可能与SLE的发病机制有关。
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