Marsh Göran, Athanasiadou Maria, Bergman Ake, Asplund Lillemor
Department of Environmental Chemistry, Institute of Applied Environmental Research, Stockholm University, SE-106 91 Stockholm, Sweden.
Environ Sci Technol. 2004 Jan 1;38(1):10-8. doi: 10.1021/es034671j.
Methoxylated and hydroxylated polybrominated diphenyl ethers (MeO-PBDEs and OH-PBDEs) have recently been reported to be present in wildlife from Northern Europe. The structures of a majority of these compounds have however been unknown. In the present study, nine OH-PBDEs and six MeO-PBDEs were identified in Baltic Sea salmon (Salmo salar) blood. All OH- and MeO-PBDEs identified were substituted with four or five bromines, and five of these had one chlorine substituent. Fourteen of the OH- and MeO-PBDEs have the methoxy or hydroxy group substituted in the ortho position to the diphenyl ether bond. Identification was done by comparison of relative retention times of authentic reference standards with compounds present in salmon plasma on two gas chromatographic columns of different polarities. The identification was supported by comparisons of full-scan mass spectrometric data: electron ionization (EI) and electron capture negative ionization (ECNI). Nine of the 15 OH- and MeO-PBDEs identified have not previously been reported to occur in the environment. The structures of several identified OH- and MeO-PBDEs support natural origin. However, at least one of the OH-PBDEs may be a hydroxylated metabolite of anthropogenic polybrominated diphenyl ether (PBDE).
最近有报道称，北欧野生动物体内存在甲氧基化和羟基化多溴二苯醚（MeO - PBDEs和OH - PBDEs）。然而，这些化合物中大多数的结构尚不清楚。在本研究中，在波罗的海鲑鱼（Salmo salar）血液中鉴定出了9种OH - PBDEs和6种MeO - PBDEs。所有鉴定出的OH - PBDEs和MeO - PBDEs都被四个或五个溴原子取代，其中五种还含有一个氯取代基。14种OH - PBDEs和MeO - PBDEs的甲氧基或羟基位于二苯醚键的邻位。通过比较真实参考标准品与鲑鱼血浆中化合物在两根不同极性气相色谱柱上的相对保留时间进行鉴定。通过全扫描质谱数据（电子电离（EI）和电子捕获负电离（ECNI））的比较来支持鉴定结果。所鉴定出的15种OH - PBDEs和MeO - PBDEs中有9种此前尚未报道在环境中出现。几种鉴定出的OH - PBDEs和MeO - PBDEs的结构支持其天然来源。然而，至少有一种OH - PBDEs可能是人为多溴二苯醚（PBDE）的羟基化代谢产物。
