Zabet-Moghaddam Masoud, Heinzle Elmar, Tholey Andreas
Technische Biochemie, Universität des Saarlandes, 66123 Saarbrücken, Germany.
Rapid Commun Mass Spectrom. 2004;18(2):141-8. doi: 10.1002/rcm.1293.
A major problem hampering the use of matrix-assisted laser desorption/ionization (MALDI) mass spectrometry for quantitative measurements is the inhomogeneous distribution of analytes and matrices in solid sample preparations. The use of ionic liquids as matrices for the qualitative and quantitative analysis of low molecular weight compounds like amino acids, sugars and vitamins was investigated. The ionic liquid matrices are composed of equimolar combinations of classical MALDI matrices (sinapinic acid, alpha-cyano-4-hydroxycinnamic acid or 2,5-dihydroxybenzoic acid) with organic bases. These matrix systems allow a homogenous sample preparation with a thin ionic liquid layer having negligible vapour pressure. This leads to a facilitated qualitative and quantitative measurement of the analytes compared with classical solid matrices.
阻碍基质辅助激光解吸/电离（MALDI）质谱用于定量测量的一个主要问题是，在固体样品制备中分析物和基质的分布不均匀。研究了使用离子液体作为基质对氨基酸、糖和维生素等低分子量化合物进行定性和定量分析。离子液体基质由经典MALDI基质（芥子酸、α-氰基-4-羟基肉桂酸或2,5-二羟基苯甲酸）与有机碱的等摩尔组合组成。这些基质系统允许制备均匀的样品，形成一层蒸气压可忽略不计的薄离子液体层。与经典的固体基质相比，这使得对分析物的定性和定量测量更加容易。
