Darbre P D, Aljarrah A, Miller W R, Coldham N G, Sauer M J, Pope G S
Division of Cell and Molecular Biology, School of Animal and Microbial Sciences, University of Reading, Reading RG6 6AJ, UK.
J Appl Toxicol. 2004 Jan-Feb;24(1):5-13. doi: 10.1002/jat.958.
Parabens are used as preservatives in many thousands of cosmetic, food and pharmaceutical products to which the human population is exposed. Although recent reports of the oestrogenic properties of parabens have challenged current concepts of their toxicity in these consumer products, the question remains as to whether any of the parabens can accumulate intact in the body from the long-term, low-dose levels to which humans are exposed. Initial studies reported here show that parabens can be extracted from human breast tissue and detected by thin-layer chromatography. More detailed studies enabled identification and measurement of mean concentrations of individual parabens in samples of 20 human breast tumours by high-pressure liquid chromatography followed by tandem mass spectrometry. The mean concentration of parabens in these 20 human breast tumours was found to be 20.6 +/- 4.2 ng x g(-1) tissue. Comparison of individual parabens showed that methylparaben was present at the highest level (with a mean value of 12.8 +/- 2.2 ng x g(-1) tissue) and represents 62% of the total paraben recovered in the extractions. These studies demonstrate that parabens can be found intact in the human breast and this should open the way technically for more detailed information to be obtained on body burdens of parabens and in particular whether body burdens are different in cancer from those in normal tissues.
对羟基苯甲酸酯被用作数千种化妆品、食品和药品的防腐剂，人类会接触到这些产品。尽管最近有关对羟基苯甲酸酯雌激素特性的报告对其在这些消费品中毒性的现有概念提出了挑战，但对于人类长期接触的低剂量对羟基苯甲酸酯是否会有任何一种完整地在体内蓄积的问题依然存在。此处报告的初步研究表明，对羟基苯甲酸酯可从人类乳腺组织中提取出来，并通过薄层色谱法进行检测。更详细的研究通过高压液相色谱-串联质谱联用技术，对20例人类乳腺肿瘤样本中各对羟基苯甲酸酯的平均浓度进行了鉴定和测量。发现这20例人类乳腺肿瘤中对羟基苯甲酸酯的平均浓度为20.6±4.2 ng x g(-1)组织。对各对羟基苯甲酸酯的比较表明，对羟基苯甲酸甲酯的含量最高（平均值为12.8±2.2 ng x g(-1)组织），占提取物中回收的对羟基苯甲酸酯总量的62%。这些研究表明，在人类乳腺中可发现完整的对羟基苯甲酸酯，这在技术上应为获取有关对羟基苯甲酸酯体内负荷的更详细信息，特别是癌症患者与正常组织患者的体内负荷是否存在差异开辟道路。
