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人血管生成素的结晶及初步X射线分析

Crystallization and preliminary X-ray analysis of human angiogenin.

作者信息

Acharya K R, Subramanian V, Shapiro R, Riordan J F, Vallee B L

机构信息

Department of Biochemistry, University of Bath, Claverton Down, U.K.

出版信息

J Mol Biol. 1992 Dec 20;228(4):1269-70. doi: 10.1016/0022-2836(92)90333-f.

Abstract

Crystals of recombinant human angiogenin have been grown from solutions containing sodium potassium tartrate and polyethylene glycol as precipitants. They belong to the space group C222(1) (a = 83.36 A, b = 120.64 A, c = 37.72 A) and contain a single molecule in the asymmetric unit. The crystals diffract to at least 2.3 A resolution and are suitable for three-dimensional X-ray structural analysis.

摘要

重组人血管生成素晶体是从含有酒石酸钠钾和聚乙二醇作为沉淀剂的溶液中生长出来的。它们属于空间群C222(1)(a = 83.36 Å,b = 120.64 Å,c = 37.72 Å),不对称单位中包含一个分子。这些晶体的衍射分辨率至少为2.3 Å,适用于三维X射线结构分析。

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