胆固醇 - 磷脂单层表面荧光中的对比度反转
Contrast inversion in the epifluorescence of cholesterol-phospholipid monolayers.
作者信息
Okonogi T M, McConnell H M
机构信息
Department of Chemistry, Stanford University, Stanford, California 94305-5080, USA.
出版信息
Biophys J. 2004 Feb;86(2):880-90. doi: 10.1016/S0006-3495(04)74163-6.
A number of dihydrocholesterol-phospholipid mixtures have been examined using the epifluorescence microscopy of monolayers at the air-water interface. These mixtures form two coexisting liquids. Fluorescence contrast was provided using a variety of different lipid probes. With increasing monolayer pressure, all of the charged probes show contrast inversion at higher dihydrocholesterol concentrations. That is, with increasing pressure the charged probes transfer from one liquid to the other, reversing the fluorescence contrast. A wide variety of phospholipids were studied, and the inversion was seen in all cases. In the inverted state and at the higher dihydrocholesterol concentrations, the immiscibility persists to the highest pressures employed, 30-40 mN/m. The data show that binary dihydrocholesterol-phospholipid mixtures can form three distinct liquids, one of which is interpreted as a phase rich in condensed complex.
已使用空气-水界面单层的落射荧光显微镜检查了多种二氢胆固醇-磷脂混合物。这些混合物形成两种共存液体。使用各种不同的脂质探针提供荧光对比度。随着单层压力增加，所有带电探针在较高二氢胆固醇浓度下均显示对比度反转。也就是说，随着压力增加，带电探针从一种液体转移到另一种液体，使荧光对比度反转。研究了多种磷脂，在所有情况下均观察到反转。在反转状态和较高二氢胆固醇浓度下，不混溶性持续到所采用的最高压力，即30-40 mN/m。数据表明，二元二氢胆固醇-磷脂混合物可形成三种不同的液体，其中一种被解释为富含凝聚复合物的相。
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