Zafar Tasleem A, Weaver Connie M, Zhao Yongdong, Martin Berdine R, Wastney Meryl E
Foods and Nutrition, Purdue University, West Lafayette, IN 47907, USA.
J Nutr. 2004 Feb;134(2):399-402. doi: 10.1093/jn/134.2.399.
Nondigestible oligosaccharides (NDO) including inulin and fructooligosaccharides (FOS) have been reported to stimulate calcium absorption. Here we report the effect of a mixture of inulin and FOS (Raftilose Synergy 1, Orafti) on calcium and bone metabolism in ovariectomized (OVX) rats. OVX rats (6 mo old) were fed a semipurified diet for 3 mo in our animal care laboratory for stabilization after ovariectomy. They were then divided into two groups (n = 13/group) and fed either a control or a NDO-supplemented diet (55 g/kg) for 21 d. Catheters were placed in their jugular veins. After 2 d, a tracer ((45)Ca) was administered by gavage or i.v. and blood was sampled for up to 300 min. Urine and fecal samples were collected for 4 d after (45)Ca administration. Femurs were measured for bone mineral density (BMD), breaking strength, and total calcium. Calcium absorption, femoral calcium content, BMD, and bone balance (V(bal)) were significantly increased (P < 0.05) by NDO, whereas the bone resorption rate relative to the bone formation rate was significantly depressed by NDO. We conclude that feeding NDO at 5.5 g/100 g for 21 d has a positive effect on calcium absorption and retention in ovariectomized rats.
据报道，包括菊粉和低聚果糖（FOS）在内的难消化性寡糖（NDO）可刺激钙的吸收。在此，我们报告菊粉和FOS混合物（Raftilose Synergy 1，益力多公司）对去卵巢（OVX）大鼠钙和骨代谢的影响。6月龄的OVX大鼠在我们的动物饲养实验室中喂食半纯化日粮3个月，以便在去卵巢后使其稳定。然后将它们分为两组（每组n = 13），并分别喂食对照日粮或添加NDO的日粮（55 g/kg）21天。将导管插入它们的颈静脉。2天后，通过灌胃或静脉注射给予示踪剂（45Ca），并在长达300分钟内采集血液样本。在给予45Ca后收集4天的尿液和粪便样本。测量股骨的骨矿物质密度（BMD）、断裂强度和总钙含量。NDO可显著提高钙吸收、股骨钙含量、BMD和骨平衡（V(bal)）（P < 0.05），而NDO可显著降低相对于骨形成率的骨吸收速率。我们得出结论，在去卵巢大鼠中，以5.5 g/100 g的量喂食NDO 21天对钙的吸收和保留具有积极作用。
