Division of Microbiology, Brewing and Biotechnology, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, UK.
Saputo Dairy UK, Innovation Centre, Harper Adams University, Newport TF10 8NB, UK.
Nutrients. 2020 Apr 30;12(5):1281. doi: 10.3390/nu12051281.
Prebiotic oligosaccharides are widely used as human and animal feed additives for their beneficial effects on the gut microbiota. However, there are limited data to assess the direct effect of such functional foods on the transcriptome of intestinal epithelial cells. The purpose of this study is to describe the differential transcriptomes and cellular pathways of colonic cells directly exposed to galacto-oligosaccharides (GOS) and fructo-oligosaccharides (FOS). We have examined the differential gene expression of polarized Caco-2 cells treated with GOS or FOS products and their respective mock-treated cells using mRNA sequencing (RNA-seq). A total of 89 significant differentially expressed genes were identified between GOS and mock-treated groups. For FOS treatment, a reduced number of 12 significant genes were observed to be differentially expressed relative to the control group. KEGG and gene ontology functional analysis revealed that genes up-regulated in the presence of GOS were involved in digestion and absorption processes, fatty acids and steroids metabolism, potential antimicrobial proteins, energy-dependent and -independent transmembrane trafficking of solutes and amino acids. Using our data, we have established complementary non-prebiotic modes of action for these frequently used dietary fibers.
益生元低聚糖因其对肠道微生物群的有益作用而被广泛用作人和动物的饲料添加剂。然而，评估此类功能性食品对肠道上皮细胞转录组的直接影响的数据有限。本研究的目的是描述直接暴露于半乳糖低聚糖（GOS）和果寡糖（FOS）的结肠细胞的差异转录组和细胞途径。我们使用 mRNA 测序（RNA-seq）检查了用 GOS 或 FOS 产品及其相应模拟处理的极化 Caco-2 细胞的差异基因表达。在 GOS 与模拟处理组之间鉴定出 89 个显著差异表达的基因。对于 FOS 处理，与对照组相比，观察到数量减少的 12 个显著差异表达的基因。KEGG 和基因本体功能分析表明，在 GOS 存在下上调的基因参与消化和吸收过程、脂肪酸和类固醇代谢、潜在的抗菌蛋白、能量依赖和非依赖的溶质和氨基酸的跨膜转运。使用我们的数据，我们为这些常用膳食纤维建立了互补的非益生元作用模式。
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