一种用于检测人参皂苷Rb1和Rg1的一步式免疫层析测定法。

A one-step immunochromatographic assay for detecting ginsenosides Rb1 and Rg1.

作者信息

Putalun Waraporn, Fukuda Noriko, Tanaka Hiroyuki, Shoyama Yukihiro

机构信息

Faculty of Pharmaceutical Sciences, Khon Kaen University, 40002, Khon Kaen, Thailand.

出版信息

Anal Bioanal Chem. 2004 Mar;378(5):1338-41. doi: 10.1007/s00216-003-2408-x. Epub 2004 Jan 28.

DOI:10.1007/s00216-003-2408-x

PMID:14747899

Abstract

An immunochromatographic strip test has been developed for detecting ginsenosides Rb1 (G-Rb1) and Rg1 (G-Rg1). This qualitative assay system is useful as a rapid screening method for detecting G-Rb1 and G-Rg1 in plants and plant preparations. Our assay is a competitive immunoassay that uses anti-G-Rb1 and anti-G-Rg1 monoclonal antibodies (MAbs) and a detection reagent that contains colloidal gold particles coated with anti-G-Rb1 and anti-G-Rg1 MAbs. Detection limits are 2 microg mL(-1) for both G-Rb1 and G-Rg1.

摘要

已开发出一种免疫层析试纸条检测方法，用于检测人参皂苷Rb1（G-Rb1）和Rg1（G-Rg1）。这种定性检测系统作为一种快速筛选方法，可用于检测植物及植物制品中的G-Rb1和G-Rg1。我们的检测方法是一种竞争性免疫分析，使用抗G-Rb1和抗G-Rg1单克隆抗体（MAbs）以及一种检测试剂，该试剂含有包被有抗G-Rb1和抗G-Rg1单克隆抗体的胶体金颗粒。G-Rb1和G-Rg1的检测限均为2微克/毫升。