Abrahão Thalita Balsamo, Freymüller Edna, Mortara Renato Arruda, Lopes José Daniel, Mariano Mario
Disciplina de Imunologia, Departamento de Microbiologia, Imunologia e Parasitologia - Universidade Federal de São Paulo, Rua Botucatu, 862 Ed. de Ciências Biomédicas 4o andar, 04023-900 São Paulo, Brazil.
Immunobiology. 2003;208(4):401-11. doi: 10.1078/0171-2985-00287.
At least three B cell subsets, B-1a, B-1b and B-2 are present circulating peripherally in the mouse. In these animals, B-1 cells constitute a minor fraction of B cells in spleen and are absent in lymph nodes although they represent the main B cell population in peritoneal and pleural cavities. Currently these cells are identified by a surface phenotypic repertoire; they express Mac-1, IgM(high), and B220(low). B-1a cells express CDS. The aim of this work emerged from the fact that the morphology of B-1 cells is not fully characterized. Here we identified B-1 cells using colloidal gold immunocytochemical assays and purified B-1 cells from supernatants of adherent peritoneal cell cultures by a magnetic bead technique. These techniques lead us to demonstrate that, in mice, either B-1a or B-1b cells have a unique morphology distinct from that of B-2 cells.
小鼠外周血中至少存在三种B细胞亚群，即B-1a、B-1b和B-2。在这些动物中，B-1细胞在脾脏B细胞中占比很小，在淋巴结中不存在，尽管它们是腹膜腔和胸膜腔中的主要B细胞群体。目前，这些细胞通过表面表型特征来识别；它们表达Mac-1、IgM（高表达）和B220（低表达）。B-1a细胞表达CD5。这项工作的目的源于B-1细胞的形态尚未完全明确这一事实。在这里，我们使用胶体金免疫细胞化学分析鉴定B-1细胞，并通过磁珠技术从贴壁腹膜细胞培养上清液中纯化B-1细胞。这些技术使我们证明，在小鼠中，B-1a或B-1b细胞具有与B-2细胞不同的独特形态。
